Abstract

In striated muscle, the thin filament consists of polymerized actin, which is decorated with the regulatory proteins troponin C (TnC) and tropomyosin. TnC is a single-polypeptide protein with a molecular weight near 18,000. We study the regulatory domain of TnC from chiken skeletal muscle using genetically generated mutants which contain a single tryptophan at positions 22, 52, and 90. The quantum yields of Trp-22 are 0.33 and 0.25 in the presence of Mg2+ (2-Mg state) and Ca2+ (4-Ca state), respectively. The anisotropy decay of of all three tryptophans show two rotational correlation times. The long correlation time observed for Trp-22 and Trp-90 suggest an asymmetric hydrodynamic shape. Data obtained at the CFS showed that TnC becomes more asymmetric upon binding the activator Ca2+.?

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR008119-09
Application #
6444725
Study Section
Project Start
2001-04-01
Project End
2002-03-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
9
Fiscal Year
2001
Total Cost
$293,069
Indirect Cost

  Institution

Name
University of Maryland Baltimore
Department
Type
DUNS #
003255213
City
Baltimore
State
MD
Country
United States
Zip Code
21201

  Publications

Piao, Wenji; Shirey, Kari Ann; Ru, Lisa W et al. (2015) A Decoy Peptide that Disrupts TIRAP Recruitment to TLRs Is Protective in a Murine Model of Influenza. Cell Rep 11:1941-52
Hartman, Kira G; McKnight, Laura E; Liriano, Melissa A et al. (2013) The evolution of S100B inhibitors for the treatment of malignant melanoma. Future Med Chem 5:97-109
Fu, Yi; Zhang, Jian; Lakowicz, Joseph R (2009) Highly efficient detection of single fluorophores in blood serum samples with high autofluorescence. Photochem Photobiol 85:646-51
Zhang, Jian; Badugu, Ramachandram; Lakowicz, Joseph R (2008) Fluorescence Quenching of CdTe Nanocrystals by Bound Gold Nanoparticles in Aqueous Solution. Plasmonics 3:3-11
Zhang, Jian; Fu, Yi; Chowdhury, Mustafa H et al. (2007) Metal-enhanced single-molecule fluorescence on silver particle monomer and dimer: coupling effect between metal particles. Nano Lett 7:2101-7
Zhang, Jian; Fu, Yi; Chowdhury, Mustafa H et al. (2007) Single-Molecule Studies on Fluorescently Labeled Silver Particles: Effects of Particle Size. J Phys Chem C Nanomater Interfaces 112:18
Zhang, Jian; Fu, Yi; Lakowicz, Joseph R (2007) Single cell fluorescence imaging using metal plasmon-coupled probe. Bioconjug Chem 18:800-5
Zhang, Jian; Fu, Yi; Lakowicz, Joseph R (2007) Emission Behavior of Fluorescently Labeled Silver Nanoshell: Enhanced Self-Quenching by Metal Nanostructure. J Phys Chem C Nanomater Interfaces 111:1955-1961
Zhang, Jian; Lakowicz, Joseph R (2007) Metal-enhanced fluorescence of an organic fluorophore using gold particles. Opt Express 15:2598-606
Fu, Yi; Lakowicz, Joseph R (2007) Single-Molecule Studies of Enhanced Fluorescence on Silver Island Films. Plasmonics 2:1-4

Showing the most recent 10 out of 44 publications