This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to test a new class of fluorescent biomarkers with strongly enhanced by metallic particles fluorescence response and develop a novel generic approach for ultra-sensitive fluorescence assay technology. The assay platform will utilize metal particles deposited on the glass/quartz surface covered with sub-nanometer layer of fluorescent biomarker. Fluorescence signal of such composite will be enormously enhanced (in many cases thousands fold). This will allow easy, quantitative and inexpensive fluorescence detection of minimal traces of specific antigens.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR008119-14
Application #
7366231
Study Section
Special Emphasis Panel (ZRG1-SSS-U (02))
Project Start
2006-04-01
Project End
2007-03-31
Budget Start
2006-04-01
Budget End
2007-03-31
Support Year
14
Fiscal Year
2006
Total Cost
$20,748
Indirect Cost
Name
University of Maryland Baltimore
Department
Biochemistry
Type
Schools of Medicine
DUNS #
188435911
City
Baltimore
State
MD
Country
United States
Zip Code
21201
Piao, Wenji; Shirey, Kari Ann; Ru, Lisa W et al. (2015) A Decoy Peptide that Disrupts TIRAP Recruitment to TLRs Is Protective in a Murine Model of Influenza. Cell Rep 11:1941-52
Hartman, Kira G; McKnight, Laura E; Liriano, Melissa A et al. (2013) The evolution of S100B inhibitors for the treatment of malignant melanoma. Future Med Chem 5:97-109
Fu, Yi; Zhang, Jian; Lakowicz, Joseph R (2009) Highly efficient detection of single fluorophores in blood serum samples with high autofluorescence. Photochem Photobiol 85:646-51
Zhang, Jian; Badugu, Ramachandram; Lakowicz, Joseph R (2008) Fluorescence Quenching of CdTe Nanocrystals by Bound Gold Nanoparticles in Aqueous Solution. Plasmonics 3:3-11
Zhang, Jian; Lakowicz, Joseph R (2007) Metal-enhanced fluorescence of an organic fluorophore using gold particles. Opt Express 15:2598-606
Fu, Yi; Lakowicz, Joseph R (2007) Single-Molecule Studies of Enhanced Fluorescence on Silver Island Films. Plasmonics 2:1-4
Ray, Krishanu; Szmacinski, Henryk; Enderlein, Joerg et al. (2007) Distance dependence of surface plasmon-coupled emission observed using Langmuir-Blodgett films. Appl Phys Lett 90:251116
Chowdhury, Mustafa H; Malyn, Stuart N; Aslan, Kadir et al. (2007) First Observation of Surface Plasmon-Coupled Chemiluminescence (SPCC). Chem Phys Lett 435:114-118
Zhang, Jian; Fu, Yi; Chowdhury, Mustafa H et al. (2007) Enhanced Forster Resonance Energy Transfer on Single Metal Particle. 2. Dependence on Donor-Acceptor Separation Distance, Particle Size, and Distance from Metal Surface. J Phys Chem C Nanomater Interfaces 111:11784-11792
Zhang, Jian; Fu, Yi; Lakowicz, Joseph R (2007) Luminescent images of single gold nanoparticles and their labeling on silica beads. Opt Express 15:13415-20

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