This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dr. Roll has investigated the use of silver and gold colloids as possible biosensors to detect glucose and proteins of biological interest. One approach has used a biotin linked bovine serum albumin gold colloid system to quantify protein binding and aggregation by avidin. This aggregation is measured by a shift in the surface plasmon resonance of the gold colloid. Gold colloids linked to maltose and dextran have also been prepared to detect binding and aggregation by the lectin protein Concanavalin A. This type of aggregation can be used to measure glucose levels by quantifying the inhibition of aggregation in the presence of varying concentrations of glucose. Silver and gold colloid systems may also be used to detect metal enhanced fluorescence. By attaching fluorescent labels to various biomolecules covalently bound to the gold and silver colloids, one can measure fluorescence as a function of colloid aggregation.
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