This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The development of BioCAT's microprobe capabilities has been proposed for the current NIH grant cycle. A thrust area at the BioCAT facility is static and time-resolved fiber-diffraction measurements from complex biological materials. One of these areas is the structure of collagenous fibers within small ordered regions in tendons, cartilage, and skin. Here we report on preliminary microdiffraction experiments demonstrating the feasibility of such studies on 18ID. A KB mirror system (Xradia Inc.) was used to produce a 6x6 um^2 focused beam, 300 mm from the last mirror, and the incident beam was collimated to 100x100 microns to reduce the divergence. The system was aligned to a 12 keV monochromatic beam from our Si(111) monochromator. The beam size obtained was 6 x 6 micron FWHM at the sample position (20 cm from the second KB mirror). The samples were kept at low temperature using a cryostream to prevent radiation damage. We were able to resolve the closely spaced (1/67 mm) collagen meridional reflections from rat-tail collagen (type I) and from cartilage tissue (type II). We anticipate that this setup will be a versatile tool for studying collagen-based structures in a number of connective tissue types, as well as open up the possibility of studying other locally ordered structures (e.g. Amyloid aggregates) in thin tissue specimens.
| Sullivan, Brendan; Robison, Gregory; Pushkar, Yulia et al. (2017) Copper accumulation in rodent brain astrocytes: A species difference. J Trace Elem Med Biol 39:6-13 |
| Orgel, Joseph P R O; Sella, Ido; Madhurapantula, Rama S et al. (2017) Molecular and ultrastructural studies of a fibrillar collagen from octocoral (Cnidaria). J Exp Biol 220:3327-3335 |
| Yazdi, Aliakbar Khalili; Vezina, Grant C; Shilton, Brian H (2017) An alternate mode of oligomerization for E. coli SecA. Sci Rep 7:11747 |
| Morris, Martha Clare (2016) Nutrition and risk of dementia: overview and methodological issues. Ann N Y Acad Sci 1367:31-7 |
| Robison, Gregory; Sullivan, Brendan; Cannon, Jason R et al. (2015) Identification of dopaminergic neurons of the substantia nigra pars compacta as a target of manganese accumulation. Metallomics 7:748-55 |
| Gelfand, Paul; Smith, Randy J; Stavitski, Eli et al. (2015) Characterization of Protein Structural Changes in Living Cells Using Time-Lapsed FTIR Imaging. Anal Chem 87:6025-31 |
| Liang, Wenguang G; Ren, Min; Zhao, Fan et al. (2015) Structures of human CCL18, CCL3, and CCL4 reveal molecular determinants for quaternary structures and sensitivity to insulin-degrading enzyme. J Mol Biol 427:1345-1358 |
| Zhou, Hao; Li, Shangyang; Badger, John et al. (2015) Modulation of HIV protease flexibility by the T80N mutation. Proteins 83:1929-39 |
| Nobrega, R Paul; Arora, Karunesh; Kathuria, Sagar V et al. (2014) Modulation of frustration in folding by sequence permutation. Proc Natl Acad Sci U S A 111:10562-7 |
| Jiao, Lianying; Ouyang, Songying; Shaw, Neil et al. (2014) Mechanism of the Rpn13-induced activation of Uch37. Protein Cell 5:616-30 |
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