This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this project is to test the feasibility of TiO2 and derivative nanoparticles (Co-Fe-TiO2 and Co-Fe-Au-TiO2) nanoparticles and nanoparticle bio-organic molecule nanocomposites as diagnostic and/or therapeutic vehicles. Our previous experiments in cultured cells have shown that TiO2-DNA nanocomposites have unique and novel biochemical properties that would be very suitable for biomedical uses (Paunesku, et al.. Nature Materials (2003) 2 (5):343-346). We have since developed several new nanoparticles, and we investigated different ways to induce cellular uptake of such nanoparticles/nanocomposites (Figure 1). Therefore, we are now poised to test these different nano-constructs in vivo, in mice. Our overall goals can be organized in two aims:
Aim 1) Testing different nanoparticle derivatives of TiO2 for in vivo stability and distribution Aim 2) Testing different nanoparticle coatings for in vivo stability and establishing their effects on in vivo distribution. At the BioCAT beamline we are investigating presence of titanium, cobalt, iron, gold and relevant """"""""biological"""""""" elements: P, S, Ca, Zn using X-ray fluorescence in different mouse tissue sections with large surface area. We inject the nanocomposites directly into tumor tissue or intravenously or intraperitoneally. We subsequently isolate different tissues, freeze and cryosection them. We look for overlap of Ti signal with fluorescence signals of other elements present in nanoparticles and map their presence in different areas of tissue by comparing these signals with the signals produced by P, S, Zn and other elements """"""""naturally"""""""" present in tissues.
| Orgel, Joseph P R O; Sella, Ido; Madhurapantula, Rama S et al. (2017) Molecular and ultrastructural studies of a fibrillar collagen from octocoral (Cnidaria). J Exp Biol 220:3327-3335 |
| Yazdi, Aliakbar Khalili; Vezina, Grant C; Shilton, Brian H (2017) An alternate mode of oligomerization for E. coli SecA. Sci Rep 7:11747 |
| Sullivan, Brendan; Robison, Gregory; Pushkar, Yulia et al. (2017) Copper accumulation in rodent brain astrocytes: A species difference. J Trace Elem Med Biol 39:6-13 |
| Morris, Martha Clare (2016) Nutrition and risk of dementia: overview and methodological issues. Ann N Y Acad Sci 1367:31-7 |
| Robison, Gregory; Sullivan, Brendan; Cannon, Jason R et al. (2015) Identification of dopaminergic neurons of the substantia nigra pars compacta as a target of manganese accumulation. Metallomics 7:748-55 |
| Gelfand, Paul; Smith, Randy J; Stavitski, Eli et al. (2015) Characterization of Protein Structural Changes in Living Cells Using Time-Lapsed FTIR Imaging. Anal Chem 87:6025-31 |
| Liang, Wenguang G; Ren, Min; Zhao, Fan et al. (2015) Structures of human CCL18, CCL3, and CCL4 reveal molecular determinants for quaternary structures and sensitivity to insulin-degrading enzyme. J Mol Biol 427:1345-1358 |
| Zhou, Hao; Li, Shangyang; Badger, John et al. (2015) Modulation of HIV protease flexibility by the T80N mutation. Proteins 83:1929-39 |
| Nobrega, R Paul; Arora, Karunesh; Kathuria, Sagar V et al. (2014) Modulation of frustration in folding by sequence permutation. Proc Natl Acad Sci U S A 111:10562-7 |
| Jiao, Lianying; Ouyang, Songying; Shaw, Neil et al. (2014) Mechanism of the Rpn13-induced activation of Uch37. Protein Cell 5:616-30 |
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