Abstract

The aim of this project is to investigate the transport of liposome entrapped substances into the skin and to the inner layers of the skin, by EPR in vivo and to approach the actual conditions which are used in local application of liposomes on humans. These experiments will contribute to the optimization of liposomal formulations for topical application on humans in dermatology, in skin care, and in protection of the skin by liposomes. For this purpose different nitroxides will be entrapped in the liposomes and the EPR spectra intensity decay will be measured with time after application of liposomes on skin or after intradermal injection of liposomes. The intensity decay is a consequence of liposome disintegration, reduction of nitroxides on the skin or inside the skin, and clearance of nitroxide by blood flow. We postulate that with in vivo EPR information can be obtained about the stability of liposomes in contact with skin and about their transport into the skin and within the skin.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
1P41RR011602-01A1
Application #
5225989
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Sinha, Birandra K; Leinisch, Fabian; Bhattacharjee, Suchandra et al. (2014) DNA cleavage and detection of DNA radicals formed from hydralazine and copper (II) by ESR and immuno-spin trapping. Chem Res Toxicol 27:674-82
Dunn, Jeff F; Khan, Mohammad N; Hou, Huagang G et al. (2011) Cerebral oxygenation in awake rats during acclimation and deacclimation to hypoxia: an in vivo electron paramagnetic resonance study. High Alt Med Biol 12:71-7
Shen, Jiangang; Khan, Nadeem; Lewis, Lionel D et al. (2003) Oxygen consumption rates and oxygen concentration in molt-4 cells and their mtDNA depleted (rho0) mutants. Biophys J 84:1291-8
Khan, Nadeem; Wilmot, Carmen M; Rosen, Gerald M et al. (2003) Spin traps: in vitro toxicity and stability of radical adducts. Free Radic Biol Med 34:1473-81
Pogue, Brian W; O'Hara, Julia A; Demidenko, Eugene et al. (2003) Photodynamic therapy with verteporfin in the radiation-induced fibrosarcoma-1 tumor causes enhanced radiation sensitivity. Cancer Res 63:1025-33
Hou, Huagang; Grinberg, Oleg Y; Taie, Satoshi et al. (2003) Electron paramagnetic resonance assessment of brain tissue oxygen tension in anesthetized rats. Anesth Analg 96:1467-72, table of contents
Chen, Bin; Pogue, Brian W; Goodwin, Isak A et al. (2003) Blood flow dynamics after photodynamic therapy with verteporfin in the RIF-1 tumor. Radiat Res 160:452-9
Dunn, Jeff F; Swartz, Harold M (2003) In vivo electron paramagnetic resonance oximetry with particulate materials. Methods 30:159-66
Khan, Nadeem; Shen, Jiangang; Chang, Ta Yuan et al. (2003) Plasma membrane cholesterol: a possible barrier to intracellular oxygen in normal and mutant CHO cells defective in cholesterol metabolism. Biochemistry 42:23-9
Swartz, Harold M (2002) Measuring real levels of oxygen in vivo: opportunities and challenges. Biochem Soc Trans 30:248-52

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