Abstract

Although nitric oxide (NO) is a central mediator during endotoxin-induced sepsis, direct detection of tissue NO in vivo, has until recently been difficult, and techniques have relied on indirect measurement of byproducts in blood or invasive technology. We have utilized electron paramagnetic resonance (EPR) in conjunction with the spin-trapping technique to detect NO directly, and non-invasively, from the tissue of septic mice. Relative signal intensity arising from NO complexed with iron and diethyldithiocarbamate (DETC) measured directly from the liver and kidney of mice given endotoxin was maximal at 6 hours post endotoxin. The quality of the EPR signal obtained (high signal to noise ratio of 15:1) using our experimental set-up and L-band EPR hardware was such that we were able to establish a time course of NO production in tissue following endotoxin, and measurement of NO from other organs (kidney and spleen). This technique was extended to experiments in which we first implanted an oxygen sensitive material (gloxy) into the liver of mice, and then monitored NO production following endotoxin. Due to the fact that the EPR spectrum from gloxy and that of NO-Fe-(DETC)2 do not overlap, we were able to monitor NO production and pO2 simulatneously in tissue, in real time.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR011602-05
Application #
6353189
Study Section
Project Start
2000-09-01
Project End
2002-04-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
5
Fiscal Year
2000
Total Cost
$12,155
Indirect Cost

  Institution

Name
Dartmouth College
Department
Type
DUNS #
041027822
City
Hanover
State
NH
Country
United States
Zip Code
03755

  Publications

Sinha, Birandra K; Leinisch, Fabian; Bhattacharjee, Suchandra et al. (2014) DNA cleavage and detection of DNA radicals formed from hydralazine and copper (II) by ESR and immuno-spin trapping. Chem Res Toxicol 27:674-82
Dunn, Jeff F; Khan, Mohammad N; Hou, Huagang G et al. (2011) Cerebral oxygenation in awake rats during acclimation and deacclimation to hypoxia: an in vivo electron paramagnetic resonance study. High Alt Med Biol 12:71-7
Shen, Jiangang; Khan, Nadeem; Lewis, Lionel D et al. (2003) Oxygen consumption rates and oxygen concentration in molt-4 cells and their mtDNA depleted (rho0) mutants. Biophys J 84:1291-8
Khan, Nadeem; Wilmot, Carmen M; Rosen, Gerald M et al. (2003) Spin traps: in vitro toxicity and stability of radical adducts. Free Radic Biol Med 34:1473-81
Pogue, Brian W; O'Hara, Julia A; Demidenko, Eugene et al. (2003) Photodynamic therapy with verteporfin in the radiation-induced fibrosarcoma-1 tumor causes enhanced radiation sensitivity. Cancer Res 63:1025-33
Hou, Huagang; Grinberg, Oleg Y; Taie, Satoshi et al. (2003) Electron paramagnetic resonance assessment of brain tissue oxygen tension in anesthetized rats. Anesth Analg 96:1467-72, table of contents
Chen, Bin; Pogue, Brian W; Goodwin, Isak A et al. (2003) Blood flow dynamics after photodynamic therapy with verteporfin in the RIF-1 tumor. Radiat Res 160:452-9
Dunn, Jeff F; Swartz, Harold M (2003) In vivo electron paramagnetic resonance oximetry with particulate materials. Methods 30:159-66
Khan, Nadeem; Shen, Jiangang; Chang, Ta Yuan et al. (2003) Plasma membrane cholesterol: a possible barrier to intracellular oxygen in normal and mutant CHO cells defective in cholesterol metabolism. Biochemistry 42:23-9
James, Philip E; Madhani, Melanie; Roebuck, William et al. (2002) Endotoxin-induced liver hypoxia: defective oxygen delivery versus oxygen consumption. Nitric Oxide 6:18-28

Showing the most recent 10 out of 44 publications