Abstract

Examine the effects of alpha particles on the overall genetic integrity of mammalian cells, to establish the minimal number of alpha particles necessary for the transformation of individual mammalian cells, and to identify and compare the genetic lesions (activation of oncogenes or inactivation of tumor suppressor genes) induced in these transformants. To date, a genetic signature for mammalian cells that have been transformed by ionizing radiation has yet to be determined. The results generated from these proposed studies will provide important clues into defining the mutational spectrum induced by single, or multiple alpha particles in mammalian cells at the DNA level, and how these mutations lead to cellular transformation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
1P41RR011623-01A1
Application #
5226000
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Chai, Y; Calaf, G M; Zhou, H et al. (2013) Radiation induced COX-2 expression and mutagenesis at non-targeted lung tissues of gpt delta transgenic mice. Br J Cancer 108:91-8
Chai, Y; Lam, R K K; Calaf, G M et al. (2013) Radiation-induced non-targeted response in vivo: role of the TGFýý-TGFBR1-COX-2 signalling pathway. Br J Cancer 108:1106-12
Hu, Burong; Grabham, Peter; Nie, Jing et al. (2012) Intrachromosomal changes and genomic instability in site-specific microbeam-irradiated and bystander human-hamster hybrid cells. Radiat Res 177:25-34
Hei, Tom K; Zhao, Yongliang; Zhou, Hongning et al. (2011) Mechanism of radiation carcinogenesis: role of the TGFBI gene and the inflammatory signaling cascade. Adv Exp Med Biol 720:163-70
Hei, Tom K; Ballas, Leslie K; Brenner, David J et al. (2009) Advances in radiobiological studies using a microbeam. J Radiat Res 50 Suppl A:A7-A12
Chai, Yunfei; Hei, Tom K (2008) Radiation Induced Bystander Effect in vivo. Acta Med Nagasaki 53:S65-S69
Brenner, David J (2008) The linear-quadratic model is an appropriate methodology for determining isoeffective doses at large doses per fraction. Semin Radiat Oncol 18:234-9
Hei, Tom K; Zhou, Hongning; Ivanov, Vladimir N et al. (2008) Mechanism of radiation-induced bystander effects: a unifying model. J Pharm Pharmacol 60:943-50
Ponnaiya, Brian; Jenkins-Baker, Gloria; Randers-Pherson, Gerhard et al. (2007) Quantifying a bystander response following microbeam irradiation using single-cell RT-PCR analyses. Exp Hematol 35:64-8
Hei, Tom K (2006) Cyclooxygenase-2 as a signaling molecule in radiation-induced bystander effect. Mol Carcinog 45:455-60

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