This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of our work is to understand centrosome duplication and maturation. In particular, we are interested in how new centrioles assemble and how centrioles direct the recruitment of pericentriolar material to form centrosomes. Very little is known about either of these processes because centrioles are small, essential structures that are not found in fungi. To identify functionally important centrosomal components, I initiated a fluorescence screen during my postdoctoral work, in which we screened 250 genes that had been implicated in cell division using spinning disc confocal microscopy of embryos expressing GFP-beta-tubulin and GFP-histone to simultaneously monitor chromosome segregation and the dynamics of the microtubule cytoskeleton. This strategy identified seven new genes required for centrosome function: three genes required specifically for centrosome duplication, one gene required for aster assembly, and three required for spindle assembly. Cumulatively, large-scale RNAi-based screens and fluorescence analysis combined with genetics and the characterization of the C. elegans homologs of proteins identified in other systems have led to the identification of 15 proteins that affect centrosome function. We propose a collaboration to characterize centrosomal protein complexes containing these components that have been isolated by immunoprecipitation and tandem affinity purification (TAP).

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR011823-11
Application #
7420697
Study Section
Special Emphasis Panel (ZRG1-CB-H (40))
Project Start
2006-09-20
Project End
2007-08-31
Budget Start
2006-09-20
Budget End
2007-08-31
Support Year
11
Fiscal Year
2006
Total Cost
$2,859
Indirect Cost
Name
University of Washington
Department
Biochemistry
Type
Schools of Medicine
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
Xavier, Marina Amaral; Tirloni, Lucas; Pinto, Antônio F M et al. (2018) A proteomic insight into vitellogenesis during tick ovary maturation. Sci Rep 8:4698
Hollmann, Taylor; Kim, Tae Kwon; Tirloni, Lucas et al. (2018) Identification and characterization of proteins in the Amblyomma americanum tick cement cone. Int J Parasitol 48:211-224
Stieg, David C; Willis, Stephen D; Ganesan, Vidyaramanan et al. (2018) A complex molecular switch directs stress-induced cyclin C nuclear release through SCFGrr1-mediated degradation of Med13. Mol Biol Cell 29:363-375
Seixas, Adriana; Alzugaray, María Fernanda; Tirloni, Lucas et al. (2018) Expression profile of Rhipicephalus microplus vitellogenin receptor during oogenesis. Ticks Tick Borne Dis 9:72-81
Wang, Zheng; Wu, Catherine; Aslanian, Aaron et al. (2018) Defective RNA polymerase III is negatively regulated by the SUMO-Ubiquitin-Cdc48 pathway. Elife 7:
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Jin, Meiyan; Fuller, Gregory G; Han, Ting et al. (2017) Glycolytic Enzymes Coalesce in G Bodies under Hypoxic Stress. Cell Rep 20:895-908
Ogami, Koichi; Richard, Patricia; Chen, Yaqiong et al. (2017) An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression. Genes Dev 31:1257-1271
Ju Lee, Hyun; Bartsch, Deniz; Xiao, Cally et al. (2017) A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells. Nat Commun 8:1456

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