This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Still unknown in any eukaryote is a full understanding of how cells grow in an asymmetric or polarized manner, a process essential for life and/or the ability to propagate species. To date, most research has focused on understanding the intracellular signaling events that initiate and maintain polarized cell growth. The research proposed herein examines the opposite side of this cellular process. Bud formation in S. cerevisiae (Baker s yeast), a eukaryote of low cellular complexity, is used to identify intracellular signaling events that negatively regulate polarized cell growth. Particular attention is given to signaling events that result from protein interactions with Zds proteins, which are essential for negatively regulating polarized cell growth, in S. cerevisiae. Proteins that interact with Zds2p to regulate polarized cell growth are identified with a candidate-based approach that utilizes differential two-hybrid analysis. The results from this analysis, performed in collaboration with the Yeast Resource Center, will be used to direct subsequent biochemical and cytological analyses aimed at deciphering the mechanisms of polarized cell growth in eukaryotes.
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