This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Adr1 is a carbon source-responsive zinc-finger transcription factor, required for transcription of a number of glucose-repressed genes (peroxisomal protein genes, genes required for ethanol, glycerol, and fatty acid utilization). Since Adr1 is an activator of transcription (it has 4 well defined activation domains), it can t be used in a conventional 2H as a bait. We would like to make GBD fusions with specific regions of Adr1 that have been shown to be involved in glucose repression and are phosphorylated in vivo. Non of the screens done so far has revealed the identity of the kinase. In a global 2H screen, done in the Field s lab, there was only one protein, the Ppt1 phosphatase, identified as an interacting partner for Adr1. Using regulatory domains of Adr1 as GBD fusions will allow us not only to find new interactions but also to confirm previous results and narrow down regions of interaction. We think that screen will be of greatest potential benefit if done at different growth conditions.
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