This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Basal transcription by RNA polymerase II is controlled by cis-acting DNA sequences and trans-acting proteins at the core promoter, among which are TFIID and other general transcription factors. TFIID recognizes and binds both TATA-dependent and DPE-dependent core promoters. The DPE core promoter motif was originally identified in our lab and is conserved among metazoans.The general transcription factors that are sufficient to transcribe TATA promoters are known. These factors are insufficient to transcribe DPE promoters, which indicates that there is at least one other basal factor required for DPE transcription. The main objective of the proposed study is to use a biochemical approach to identify the basal factors that mediate DPE-dependent transcription. By using a defined in vitro transcription system with the known transcription factors and RNA polymerase II, I have identified and partially purified a DPE-specific transcription activity. I plan to purify this activity to near homogeneity to identify and characterize the specific factor/factors that mediate DPE-specific transcription.
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