This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dynamic exchange is being incorporated into the software for the Slowly Relaxing Local Structure (SRLS) model. This new dynamic process allows the nitroxide spin label to exchange positions between two or more sites where the spin label experiences different rotational rates and orderings. This project is motivated by our recent multifrequency study of T4 lysozyme dynamics where we have clearly differentiated two distinct conformers of the nitroxide tether. However, we previously were limited to considering the exchange to be static on the ESR time scale. Under this assumption it was found that different populations have to be employed to achieve acceptable spectral fits at different frequencies. We believe that this is a result of the different effective timescales of ESR at the different frequencies. Our new software will enable us to clarify these matters, and to obtain rates of conformational exchange.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR016292-09
Application #
7956617
Study Section
Special Emphasis Panel (ZRG1-BCMB-K (40))
Project Start
2009-09-01
Project End
2010-08-31
Budget Start
2009-09-01
Budget End
2010-08-31
Support Year
9
Fiscal Year
2009
Total Cost
$10,556
Indirect Cost
Name
Cornell University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
872612445
City
Ithaca
State
NY
Country
United States
Zip Code
14850
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