This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.The field of comparative proteomics strives to provide a means to study the up and down regulation in protein expression caused by environmentally or biologically driven changes to an organism. Shotgun proteomic approaches for comparative analyses between differential biological states are performed by measuring the relative abundances of proteins either by label free or stable isotopic labeling methods. Stable isotopic labeling schemes involve labeling each population with either a light or a heavy stable isotope and combining the two proteomes for simultaneous analysis. Relative quantification between the isotopically labeled populations is performed by taking a ratio of the area or the intensity of the light and heavy monoisotopic peaks from the MS or MS/MS. We have been involved in refining existing methodology and developing new approaches for comparative proteomics.
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