Abstract

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A relatively new type of O-linked glycan has been implicated in neuronal cell adhesion and migration, the O-linked mannose glycans. This project will use novel techniques to separate glycoproteins expressing O-Man glycans from brain tissue and identify these glycoproteins using mass spectrometry techniques. In addition, the Asn-linked glycans from mouse brain have been identified by MALDI-TOF MS. These are being compared to glycans found in mice that are null for the GnT-Va glycosyltransferase to determine how this enzyme regulates glycoprotein glycan expression. A GnT-Vb null mouse has now been produced and the effects of this deletion on O-Man and Asn-linked glycoproteins is now under study.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR018502-07
Application #
7955996
Study Section
Special Emphasis Panel (ZRG1-CB-L (40))
Project Start
2009-06-01
Project End
2010-05-31
Budget Start
2009-06-01
Budget End
2010-05-31
Support Year
7
Fiscal Year
2009
Total Cost
$2,534
Indirect Cost

  Institution

Name
University of Georgia
Department
Type
Organized Research Units
DUNS #
004315578
City
Athens
State
GA
Country
United States
Zip Code
30602

  Publications

Gas-Pascual, Elisabet; Ichikawa, Hiroshi Travis; Sheikh, Mohammed Osman et al. (2018) CRISPR/Cas9 and glycomics tools for Toxoplasma glycobiology. J Biol Chem :
Sheikh, M Osman; Thieker, David; Chalmers, Gordon et al. (2017) O2 sensing-associated glycosylation exposes the F-box-combining site of the Dictyostelium Skp1 subunit in E3 ubiquitin ligases. J Biol Chem 292:18897-18915
Ma, Liang; Chen, Zehua; Huang, Da Wei et al. (2016) Genome analysis of three Pneumocystis species reveals adaptation mechanisms to life exclusively in mammalian hosts. Nat Commun 7:10740
Karumbaiah, Lohitash; Enam, Syed Faaiz; Brown, Ashley C et al. (2015) Chondroitin Sulfate Glycosaminoglycan Hydrogels Create Endogenous Niches for Neural Stem Cells. Bioconjug Chem 26:2336-49
Li, Juan; Murtaugh, Michael P (2015) Functional analysis of porcine reproductive and respiratory syndrome virus N-glycans in infection of permissive cells. Virology 477:82-8
DePaoli-Roach, Anna A; Contreras, Christopher J; Segvich, Dyann M et al. (2015) Glycogen phosphomonoester distribution in mouse models of the progressive myoclonic epilepsy, Lafora disease. J Biol Chem 290:841-50
Dwyer, Chrissa A; Katoh, Toshihiko; Tiemeyer, Michael et al. (2015) Neurons and glia modify receptor protein-tyrosine phosphatase ? (RPTP?)/phosphacan with cell-specific O-mannosyl glycans in the developing brain. J Biol Chem 290:10256-73
Li, Juan; Tao, Shujuan; Orlando, Ron et al. (2015) N-glycosylation profiling of porcine reproductive and respiratory syndrome virus envelope glycoprotein 5. Virology 478:86-98
Gasimli, Leyla; Hickey, Anne Marie; Yang, Bo et al. (2014) Changes in glycosaminoglycan structure on differentiation of human embryonic stem cells towards mesoderm and endoderm lineages. Biochim Biophys Acta 1840:1993-2003
Panin, Vladislav M; Wells, Lance (2014) Protein O-mannosylation in metazoan organisms. Curr Protoc Protein Sci 75:Unit 12.12.

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