This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.As great progress is being made in the field of proteomics to identify the protein components in cell regulatory networks, understanding how these proteins are spatially and temporally regulated at the cellular level remains a significant task. Minimally invasive assays are required that can address how proteins function in their natural environment where compartmentalization and metabolite concentrations are strictly maintained. To address these issues, we propose to develop and apply imaging-based technologies to perform functional proteomics and assess the effects of cell-signaling pathways on protein dynamics in intact cells. These technologies will be broadly applicable to study a variety of protein networks. To develop these approaches, initial experiments will focus on the effects of oxidative stress, radiation, and intracellular signaling pathways on a class of proteins known as chromosomal passengers that undergo dramatic changes in localization during the cell cycle.
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