Abstract

This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Goals include investigation of the most effective techniques for 1) releasing glycans from glycoproteins from cells and tissues, 2)permethylating glycans and analyzing the permethylated compounds by mass spectrometry, and 3) separating glycoproteins and glycopeptides by silica-based lectin affinity chromatography.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR018942-03
Application #
7602912
Study Section
Special Emphasis Panel (ZRG1-BCMB-M (40))
Project Start
2007-09-01
Project End
2008-08-31
Budget Start
2007-09-01
Budget End
2008-08-31
Support Year
3
Fiscal Year
2007
Total Cost
$191,175
Indirect Cost

  Institution

Name
Indiana University Bloomington
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
006046700
City
Bloomington
State
IN
Country
United States
Zip Code
47401

  Publications

Yu, Chuan-Yih; Mayampurath, Anoop; Tang, Haixu (2013) Software tools for glycan profiling. Methods Mol Biol 951:269-76
Novotny, Milos V; Alley Jr, William R; Mann, Benjamin F (2013) Analytical glycobiology at high sensitivity: current approaches and directions. Glycoconj J 30:89-117
Balonova, Lucie; Mann, Benjamin F; Cerveny, Lukas et al. (2012) Characterization of protein glycosylation in Francisella tularensis subsp. holarctica: identification of a novel glycosylated lipoprotein required for virulence. Mol Cell Proteomics 11:M111.015016
Alley Jr, William R; Vasseur, Jacqueline A; Goetz, John A et al. (2012) N-linked glycan structures and their expressions change in the blood sera of ovarian cancer patients. J Proteome Res 11:2282-300
Isailovic, Dragan; Plasencia, Manolo D; Gaye, Maissa M et al. (2012) Delineating diseases by IMS-MS profiling of serum N-linked glycans. J Proteome Res 11:576-85
Mayampurath, Anoop M; Wu, Yin; Segu, Zaneer M et al. (2011) Improving confidence in detection and characterization of protein N-glycosylation sites and microheterogeneity. Rapid Commun Mass Spectrom 25:2007-19
Zhuang, Zexi; Mitra, Indranil; Hussein, Ahmed et al. (2011) Microchip electrophoresis of N-glycans on serpentine separation channels with asymmetrically tapered turns. Electrophoresis 32:246-53
Kang, Pilsoo; Madera, Milan; Alley Jr, William R et al. (2011) Glycomic Alterations in the Highly-abundant and Lesser-abundant Blood Serum Protein Fractions for Patients Diagnosed with Hepatocellular Carcinoma. Int J Mass Spectrom 305:185-198
Kurulugama, Ruwan T; Nachtigall, Fabiane M; Valentine, Stephen J et al. (2011) Overtone mobility spectrometry: part 4. OMS-OMS analyses of complex mixtures. J Am Soc Mass Spectrom 22:2049-60
Alley Jr, William R; Novotny, Milos V (2010) Glycomic analysis of sialic acid linkages in glycans derived from blood serum glycoproteins. J Proteome Res 9:3062-72

Showing the most recent 10 out of 81 publications