This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this core project is to develop methods for examining whole cells, identifying cellular structures, and localizing specific molecules in cells using three dimensional cryo x-ray tomography. Once we have accomplished these goals, we will focus on developing the methodology to simultaneously identify two or more different molecules in order to examine molecular co-localizations and interactions in situ. These apabilities will be enhanced by the development of correlated light and x-ray microscopy techniques, in which protein constructs tagged with green fluorescent protein (GFP) are tracked in live cells then localized at better resolution with x-ray tomography. Accomplishing these goals will provide powerful new tools for high throughput, high resolution (better than 50 nm) analyses that will complement the existing proteomics tools.
This specific aim i s divided into three separate tasks: 1) to develop the ability to identify cellular structures in a simple eukaryotic cell, the yeast Saccharomyces cerevisiae, with xray tomography;2) to develop the ability to identify cellular structures of complex eukaryotic cells using x-ray tomography;and 3) to develop methods for labeling molecules in cells using x-ray tomography.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR019664-06A1
Application #
8169710
Study Section
Special Emphasis Panel (ZRG1-BST-K (40))
Project Start
2010-06-01
Project End
2011-04-30
Budget Start
2010-06-01
Budget End
2011-04-30
Support Year
6
Fiscal Year
2010
Total Cost
$548,085
Indirect Cost
Name
University of California San Francisco
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143
McHugh, Emma; Batinovic, Steven; Hanssen, Eric et al. (2015) A repeat sequence domain of the ring-exported protein-1 of Plasmodium falciparum controls export machinery architecture and virulence protein trafficking. Mol Microbiol 98:1101-14
Do, Myan; Isaacson, Samuel A; McDermott, Gerry et al. (2015) Imaging and characterizing cells using tomography. Arch Biochem Biophys 581:111-21
Dasgupta, Sabyasachi; Auth, Thorsten; Gov, Nir S et al. (2014) Membrane-wrapping contributions to malaria parasite invasion of the human erythrocyte. Biophys J 107:43-54
Le Gros, Mark A; McDermott, Gerry; Cinquin, Bertrand P et al. (2014) Biological soft X-ray tomography on beamline 2.1 at the Advanced Light Source. J Synchrotron Radiat 21:1370-7
Smith, Elizabeth A; Cinquin, Bertrand P; Do, Myan et al. (2014) Correlative cryogenic tomography of cells using light and soft x-rays. Ultramicroscopy 143:33-40
Hanssen, Eric; Dekiwadia, Chaitali; Riglar, David T et al. (2013) Electron tomography of Plasmodium falciparum merozoites reveals core cellular events that underpin erythrocyte invasion. Cell Microbiol 15:1457-72
Smith, Elizabeth A; Cinquin, Bertrand P; McDermott, Gerry et al. (2013) Correlative microscopy methods that maximize specimen fidelity and data completeness, and improve molecular localization capabilities. J Struct Biol 184:12-20
Parkinson, Dilworth Y; Epperly, Lindsay R; McDermott, Gerry et al. (2013) Nanoimaging cells using soft X-ray tomography. Methods Mol Biol 950:457-81
Isaacson, Samuel A; Larabell, Carolyn A; Le Gros, Mark A et al. (2013) The influence of spatial variation in chromatin density determined by X-ray tomograms on the time to find DNA binding sites. Bull Math Biol 75:2093-117
McDermott, Gerry; Le Gros, Mark A; Larabell, Carolyn A (2012) Visualizing cell architecture and molecular location using soft x-ray tomography and correlated cryo-light microscopy. Annu Rev Phys Chem 63:225-39

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