The overall goals of this part of the research program are to apply molecular approaches to the development of biomarkers of exposure to some key toxic components in the environment of people living in the Aberjona region. These biomarkers will include readily accessible blood proteins and DNA from cells in blood and also protein and DNA from cells in potential target organs. The proposed research is a continuation and amplification of their earlier ongoing research in these same areas. The methods will include the most advanced approaches in mass spectrometry, cryogenic fluorescence spectroscopy, and postlabeling analysis of modified DNA. The contaminants that will be the main focus of the research will be the polycyclic aromatic hydrocarbons (PAH), representing exposure from air and food, and arsenic and chromium, representing exposure from water. Through examination of both serum albumin and histones and DNA they will try to develop both a short- and long- term scale for exposure and damage. The combined uses of 35S postlabeling and laser fluorescence and electrospray mass spectrometry will give unparalleled sensitivity and specificity to the use of biomarkers in epidemiology. Monoclonal antibodies will be added to those already made to give rapid and accurate analysis of oxidatively modified DNA. Experiments will be conducted to test the possibility of interactions between chromium and arsenic. Finally, all developed biomarkers will be employed in exposure studies on people in the Aberjona region.

Project Start
1999-04-01
Project End
2001-03-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
13
Fiscal Year
2000
Total Cost
$175,274
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Type
DUNS #
001425594
City
Cambridge
State
MA
Country
United States
Zip Code
02139
Senn, David B; Gawel, James E; Jay, Jennifer A et al. (2007) Long-term fate of a pulse arsenic input to a eutrophic lake. Environ Sci Technol 41:3062-8
Diez, Sergi; Noonan, Gregory O; MacFarlane, John K et al. (2007) Ferrous iron oxidation rates in the pycnocline of a permanently stratified lake. Chemosphere 66:1561-70
Risoul, Veronique; Richter, Henning; Lafleur, Arthur L et al. (2005) Effects of temperature and soil components on emissions from pyrolysis of pyrene-contaminated soil. J Hazard Mater 126:128-40
Coller, Hilary A; Khrapko, Konstantin; Herrero-Jimenez, Pablo et al. (2005) Clustering of mutant mitochondrial DNA copies suggests stem cells are common in human bronchial epithelium. Mutat Res 578:256-71
Pedersen, Daniel U; Durant, John L; Taghizadeh, Koli et al. (2005) Human cell mutagens in respirable airborne particles from the northeastern United States. 2. Quantification of mutagens and other organic compounds. Environ Sci Technol 39:9547-60
Durant, John L; Ivushkina, Tatiana; MacLaughlin, Kathy et al. (2004) Elevated levels of arsenic in the sediments of an urban pond: sources, distribution and water quality impacts. Water Res 38:2989-3000
Blute, Nicole Keon; Brabander, Daniel J; Hemond, Harold F et al. (2004) Arsenic sequestration by ferric iron plaque on cattail roots. Environ Sci Technol 38:6074-7
Pedersen, Daniel U; Durant, John L; Penman, Bruce W et al. (2004) Human-cell mutagens in respirable airborne particles in the northeastern United States. 1. Mutagenicity of fractionated samples. Environ Sci Technol 38:682-9
Southworth, Barbara A; Voelker, Bettina M (2003) Hydroxyl radical production via the photo-Fenton reaction in the presence of fulvic acid. Environ Sci Technol 37:1130-6
Tomita-Mitchell, Aoy; Ling, Losee Lucy; Glover, Curtis L et al. (2003) The mutational spectrum of the HPRT gene from human T cells in vivo shares a significant concordant set of hot spots with MNNG-treated human cells. Cancer Res 63:5793-8

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