This research will provide a basis for quantifying the relationship between contaminant exposure history and regulation of microbial biodegradative capabilities in natural porous media under flowing conditions representative of field scale plume contamination scenarios (low substrate concentrations and low levels of mixed electron acceptors [oxygen and nitrate]. Processes influences natural attenuation of four model compounds, representing three classes of priority contaminants (fuel hydrocarbons, chlorinated solvents, and polynuclear aromatic hydrocarbons) will be investigated. Well-characterized microbial/contaminant/porous media systems will be used to develop validated mathematical tools for the description of pollutant elution, transport, and microbial transformation. The experimental design is based on a series of integrated studies that range in scale from molecular (regulation of biodegradative pathways), to batch (kinetics of contaminant utilization), to bench-scale (flowing column studies), to field scale (stimulations of field conditions). Batch and column studies are proposed to: 1) evaluate the influence of substrate composition, porous media characteristics, and concentration exposure history of biodegradative gene expression under natural attenuation conditions; 2) investigate links between contaminant exposure history and regulation of biodegradative functions; 3) evaluate the influence of surface attachment on expression of biodegradative capabilities under natural attenuation conditions; and 4) develop state-of-the-art mathematical models to validate conceptual models, test hypotheses, and explore the implications of laboratory findings at the field scale. at the field scale. Knowledge gained from this Project pertaining to processes influencing microbial transformations will provide the foundation for improved Superfund site remediation at chlorinated solvent and hydrocarbon contaminated sites. In addition the work proposed here, in conjunction with Project 4e, will allow for development of approaches for assessment of 'alternative endpoint' clean- up standards under intrinsic and engineered remediation conditions.

Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Michigan State University
East Lansing
United States
Zip Code
Nault, Rance; Doskey, Claire M; Fader, Kelly A et al. (2018) Comparison of Hepatic NRF2 and Aryl Hydrocarbon Receptor Binding in 2,3,7,8-Tetrachlorodibenzo-p-dioxin-Treated Mice Demonstrates NRF2-Independent PKM2 Induction. Mol Pharmacol 94:876-884
Dornbos, Peter; LaPres, John J (2018) Incorporating population-level genetic variability within laboratory models in toxicology: From the individual to the population. Toxicology 395:1-8
Zhang, Shuai; Liu, Qinfu; Gao, Feng et al. (2018) Interfacial Structure and Interaction of Kaolinite Intercalated with N-methylformamide Insight from Molecular Dynamics Modeling. Appl Clay Sci 158:204-210
Fader, Kelly A; Nault, Rance; Raehtz, Sandi et al. (2018) 2,3,7,8-Tetrachlorodibenzo-p-dioxin dose-dependently increases bone mass and decreases marrow adiposity in juvenile mice. Toxicol Appl Pharmacol 348:85-98
Zhang, Shuai; Liu, Qinfu; Cheng, Hongfei et al. (2018) Mechanism Responsible for Intercalation of Dimethyl Sulfoxide in Kaolinite: Molecular Dynamics Simulations. Appl Clay Sci 151:46-53
Zhang, Qiang; Li, Jin; Middleton, Alistair et al. (2018) Bridging the Data Gap From in vitro Toxicity Testing to Chemical Safety Assessment Through Computational Modeling. Front Public Health 6:261
Fader, K A; Nault, R; Kirby, M P et al. (2018) Corrigendum to ""Convergence of hepcidin deficiency, systemic iron overloading, heme accumulation, and REV-ERB?/? activation in aryl hydrocarbon receptor-elicited hepatotoxicity"" [Toxicol. Appl. Pharmacol. 321 (2017) 1-17]. Toxicol Appl Pharmacol 344:74
Konganti, Kranti; Ehrlich, Andre; Rusyn, Ivan et al. (2018) gQTL: A Web Application for QTL Analysis Using the Collaborative Cross Mouse Genetic Reference Population. G3 (Bethesda) 8:2559-2562
Zhang, Shuai; Liu, Qinfu; Gao, Feng et al. (2018) Molecular Dynamics Simulation of Basal Spacing, Energetics, and Structure Evolution of a Kaolinite-Formamide Intercalation Complex and Their Interfacial Interaction. J Phys Chem C Nanomater Interfaces 122:3341-3349
Fader, Kelly A; Zacharewski, Timothy R (2017) Beyond the Aryl Hydrocarbon Receptor: Pathway Interactions in the Hepatotoxicity of 2,3,7,8-Tetrachlorodibenzo-p-dioxin and Related Compounds. Curr Opin Toxicol 2:36-41

Showing the most recent 10 out of 417 publications