The major goal of this project is to determine the genetic toxicity of complex chemical mixtures present in field extracts from wood preserving and oily waste sites as an indicator of their potential carcinogenicity. Field extracts will be tested in a mouse model and by a novel in vitro system for the induction of DNA damage (covalent DNA adducts), which will be assessed by the 32P-postlabeling assay. The investigators propose to (1) produce extract- and tissue-specific PAH- DNA adduct profiles and levels as genotoxic endpoints, (2) identify the sources of major PAH-DNA adducts induced by field extracts, (3) show whether polychlorinated phenols induce oxidative stress-associated bulky DNA lesions, (4) determine the relationships between genotoxic potencies of field extracts and tumor initiating activities, (5) assess possible synergistic or antagonistic interactions between chemicals in field extracts in vivo, (6) examine whether field extracts elicit reductions of hepatic I-compound levels in the mouse model, and (7) test whether, in the absence of metabolic activation, aqueous extracts of field samples induce extract-specific DNA adduct profiles and levels in vitro and whether this property represents an indicator of genotoxicity of aqueous dumpsite effluents. These studies are designed to provide qualitative and quantitative data on the toxicity of wood preserving and oily waste sites to the genetic material of a cell.

Project Start
1998-04-01
Project End
1999-03-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
10
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Texas A&M University
Department
Type
DUNS #
047006379
City
College Station
State
TX
Country
United States
Zip Code
77845
Phillips, Tracie D; Richardson, Molly; Cheng, Yi-Shing Lisa et al. (2015) Mechanistic relationships between hepatic genotoxicity and carcinogenicity in male B6C3F1 mice treated with polycyclic aromatic hydrocarbon mixtures. Arch Toxicol 89:967-77
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dela Cruz, Albert Leo N; Cook, Robert L; Dellinger, Barry et al. (2014) Assessment of environmentally persistent free radicals in soils and sediments from three Superfund sites. Environ Sci Process Impacts 16:44-52
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Theodorakis, Christopher W; Bickham, John W; Donnelly, Kirby C et al. (2012) DNA damage in cichlids from an oil production facility in Guatemala. Ecotoxicology 21:496-511
Dash, Bhagirathi; Phillips, Timothy D (2012) Molecular characterization of a catalase from Hydra vulgaris. Gene 501:144-52
Barhoumi, Rola; Mouneimne, Youssef; Ramos, Ernesto et al. (2011) Multiphoton spectral analysis of benzo[a]pyrene uptake and metabolism in a rat liver cell line. Toxicol Appl Pharmacol 253:45-56
Kelley, Matthew A; Gillespie, Annika; Zhou, Guo-Dong et al. (2011) In situ biomonitoring of caged, juvenile Chinook salmon (Oncorhynchus tshawytscha) in the Lower Duwamish Waterway. Mar Pollut Bull 62:2520-32
Rinner, Brian P; Matson, Cole W; Islamzadeh, Arif et al. (2011) Evolutionary toxicology: contaminant-induced genetic mutations in mosquitofish from Sumgayit, Azerbaijan. Ecotoxicology 20:365-76

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