A promising alternative treatment technology for accomplishing the destruction and detoxification of organic hazardous chemicals for on-site remediation of ground water is fixed-growth biological treatment engineered to optimize the performance of the white rot fungus Phanerochaete chrysosporium. P. chrysosporium has been shown to degrade various contaminants in laboratory shake cultures. In addition fixed-growth processes have been shown to considerably increase the lignin peroxidase activity that is presumed to be responsible for the degradation process. The proposed research approach is to use a contained fixed-growth fungal reactor (FGFR) treatment process at the bench and pilot scales to detoxify simulated and actual ground waters by degradation and mineralization of polycyclic aromatic hydrocarbons and pentachlorophenol at environmentally important concentrations. The proposed goal is to mineralize these contaminants to CO2 and H2O, thereby supporting the major emphasis of the Superfund Amendments and Reauthorization Act of 1986 (SARA) concerning treatment technologies that offer permanent protection of human health and the environment. Information concerning the technical feasibility, engineering optimization, design, field scale operation aspects, and cost effectiveness of the fixed-growth biological reactor using P. chrysosporium for degradation and detoxification of hazardous chemicals in ground water will be generated as a result of the proposed scope of work.

Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Utah State University
Department
Type
DUNS #
City
Logan
State
UT
Country
United States
Zip Code
84322
Kwon, S I; Anderson, A J (2001) Catalase activities of Phanerochaete chrysosporium are not coordinately produced with ligninolytic metabolism: catalases from a white-rot fungus. Curr Microbiol 42:8-11
Tatarko, M; Bumpus, J A (1997) Further studies on the inactivation by sodium azide of lignin peroxidase from Phanerochaete chrysosporium. Arch Biochem Biophys 339:200-9
Nie, G; Aust, S D (1997) Effect of calcium on the reversible thermal inactivation of lignin peroxidase. Arch Biochem Biophys 337:225-31
Sutherland, G R; Zapanta, L S; Tien, M et al. (1997) Role of calcium in maintaining the heme environment of manganese peroxidase. Biochemistry 36:3654-62
He, B; Sinclair, R; Copeland, B R et al. (1996) The structure-function relationship and reduction potentials of high oxidation states of myoglobin and peroxidase. Biochemistry 35:2413-20
Goodwin, D C; Aust, S D; Grover, T A (1996) Free radicals produced during the oxidation of hydrazines by hypochlorous acid. Chem Res Toxicol 9:1333-9
Whitwam, R; Tien, M (1996) Heterologous expression and reconstitution of fungal Mn peroxidase. Arch Biochem Biophys 333:439-46
Khindaria, A; Yamazaki, I; Aust, S D (1996) Stabilization of the veratryl alcohol cation radical by lignin peroxidase. Biochemistry 35:6418-24
Khindaria, A; Aust, S D (1996) EPR detection and characterization of lignin peroxidase porphyrin pi-cation radical. Biochemistry 35:13107-11
Koduri, R S; Whitwam, R E; Barr, D et al. (1996) Oxidation of 1,2,4,5-tetramethoxybenzene by lignin peroxidase of Phanerochaete chrysosporium. Arch Biochem Biophys 326:261-5

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