The Neural and Behavioral Toxicity Assessment (NBTA) Core will provide information concerning the cellular and neurobehavioral consequences of toxicant exposures being studied in our Superfund Center. Neurotoxicant actions will be evaluated by cell-based screening using neuronotypic PC12 cells, neural stem cells, and neuronal-glial co-cultures, to screen for developmental neurotoxicity across the entire spectrum of neurodevelopmental events, from cell replication to differentiation into specific neural and neurotransmitter phenotypes. This will provide comparative dose-effect relationships for related chemicals in each study, beneficial or harmful consequences of remediation strategies, and information on potential additive or synergistic toxic effects. The neurobehavioral screen will provide detailed assessment of cognitive and emotional functions affected by developmental toxicant exposures in test batteries that we have designed for the diverse models studied within the Center. The neurobehavioral batteries are tailored to the rodent and fish model systems used in the Center. These complementary models will be used to help the projects determine how the toxicant-induced molecular events, neurochemical disruption and neurocircuit miswiring cause persistent functional behavioral impairment. Specifically, for each project, the NBTA Core will include delineation of neurotransmitter systems and neuronal projections involved in the functional defects, as well as potential amelioration of toxicant-induced impairment as a result of different remediation strategies. The NBTA Core interacts with every project within the Center and provides a translation from the basic mechanistic studies of the individual projects to the potential impact on functional outcomes involving nervous system dysfunction. By providing a standardized approach to toxicity assessment, this core ensures that each of the projects and models shares common approaches to mechanism and outcome.

Public Health Relevance

The Neural and Behavioral Toxicity Assessment (NBTA) Core will provide service to all Projects. The in vitro tests will provide rapid assessment of cellular toxicity of the chemicals under study. The neurobehavioral tests will provide functional information concerning effects of the toxicants on cognitive and emotional function for rodents and fish. The NBTA Core will provide both mechanistic and functional information.

National Institute of Health (NIH)
National Institute of Environmental Health Sciences (NIEHS)
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
Project #
Application #
Study Section
Special Emphasis Panel (ZES1-SET-V)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Duke University
United States
Zip Code
Lefèvre, Emilie; Bossa, Nathan; Gardner, Courtney M et al. (2018) Biochar and activated carbon act as promising amendments for promoting the microbial debromination of tetrabromobisphenol A. Water Res 128:102-110
Kollitz, Erin M; Kassotis, Christopher D; Hoffman, Kate et al. (2018) Chemical Mixtures Isolated from House Dust Disrupt Thyroid Receptor ? Signaling. Environ Sci Technol :
Hartman, Jessica H; Smith, Latasha L; Gordon, Kacy L et al. (2018) Swimming Exercise and Transient Food Deprivation in Caenorhabditis elegans Promote Mitochondrial Maintenance and Protect Against Chemical-Induced Mitotoxicity. Sci Rep 8:8359
Luz, Anthony L; Kassotis, Christopher D; Stapleton, Heather M et al. (2018) The high-production volume fungicide pyraclostrobin induces triglyceride accumulation associated with mitochondrial dysfunction, and promotes adipocyte differentiation independent of PPAR? activation, in 3T3-L1 cells. Toxicology 393:150-159
Day, D B; Xiang, J; Mo, J et al. (2018) Combined use of an electrostatic precipitator and a high-efficiency particulate air filter in building ventilation systems: Effects on cardiorespiratory health indicators in healthy adults. Indoor Air 28:360-372
Slotkin, Theodore A; Ko, Ashley; Seidler, Frederic J (2018) Does growth impairment underlie the adverse effects of dexamethasone on development of noradrenergic systems? Toxicology 408:11-21
Rock, Kylie D; Horman, Brian; Phillips, Allison L et al. (2018) EDC IMPACT: Molecular effects of developmental FM 550 exposure in Wistar rat placenta and fetal forebrain. Endocr Connect 7:305-324
Weinhouse, Caren; Truong, Lisa; Meyer, Joel N et al. (2018) Caenorhabditis elegans as an emerging model system in environmental epigenetics. Environ Mol Mutagen 59:560-575
Sanders, Laurie H; Rouanet, Jeremy P; Howlett, Evan H et al. (2018) Newly Revised Quantitative PCR-Based Assay for Mitochondrial and Nuclear DNA Damage. Curr Protoc Toxicol 76:e50
Czaplicki, Lauren M; Dharia, Monika; Cooper, Ellen M et al. (2018) Evaluating the mycostimulation potential of select carbon amendments for the degradation of a model PAH by an ascomycete strain enriched from a superfund site. Biodegradation :

Showing the most recent 10 out of 291 publications