Abstract

Chromium and its compounds are major contaminants at a large number of the Superfund sites. Chromium exists in several oxidation states, among which only hexavalent form is associated with carcinogenic and other toxic effects of this metal. Superfund sites and other sources of environmental contamination usually contain chromium in different oxidation forms, which makes it difficult to assess human exposure to toxic chromium(VI) based on total levels of chromium in biological samples. We have previously determined that the most abundant form of DMA damage in chromium(VI)-exposed cells are mutagenic ternary chromium- DNA complexes containing ascorbate and amino acid/peptide ligands. In this work we propose to characterize the use of measurements of ternary adducts as a biomarker of genetic damage by chromium(VI) in human cells. Experiments will be performed to identify slowly repairable forms of chromium-DNA adducts, the role of biological repair and ligand exchange-dependent loss, the influence of co-exposure to other metals on biological characteristics of chromium-DNA adducts and to develop a more sensitive analytical methodology for the determination of major mutagenic chromium-DNA modifications.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
Project #
5P42ES013660-04
Application #
7599027
Study Section
Special Emphasis Panel (ZES1)
Project Start
Project End
Budget Start
2008-04-01
Budget End
2009-03-31
Support Year
4
Fiscal Year
2008
Total Cost
$370,388
Indirect Cost

  Institution

Name
Brown University
Department
Type
DUNS #
001785542
City
Providence
State
RI
Country
United States
Zip Code
02912

  Publications

Toral-Sánchez, E; Rangel-Mendez, J R; Hurt, Robert H et al. (2018) Novel application of magnetic nano-carbon composite as redox mediator in the reductive biodegradation of iopromide in anaerobic continuous systems. Appl Microbiol Biotechnol 102:8951-8961
Thompson, Marcella Remer; Schwartz Barcott, Donna (2018) The Role of the Nurse Scientist as a Knowledge Broker. J Nurs Scholarsh :
Spade, Daniel J; Dere, Edward; Hall, Susan J et al. (2018) All-trans retinoic acid disrupts development in ex vivo cultured fetal rat testes. I: Altered seminiferous cord maturation and testicular cell fate. Toxicol Sci :
Spade, Daniel J; Bai, Cathy Yue; Lambright, Christy et al. (2018) Validation of an automated counting procedure for phthalate-induced testicular multinucleated germ cells. Toxicol Lett 290:55-61
Sears, Clara G; Braun, Joseph M; Ryan, Patrick H et al. (2018) The association of traffic-related air and noise pollution with maternal blood pressure and hypertensive disorders of pregnancy in the HOME study cohort. Environ Int 121:574-581
Guelfo, Jennifer L; Adamson, David T (2018) Evaluation of a national data set for insights into sources, composition, and concentrations of per- and polyfluoroalkyl substances (PFASs) in U.S. drinking water. Environ Pollut 236:505-513
Guelfo, Jennifer L; Marlow, Thomas; Klein, David M et al. (2018) Evaluation and Management Strategies for Per- and Polyfluoroalkyl Substances (PFASs) in Drinking Water Aquifers: Perspectives from Impacted U.S. Northeast Communities. Environ Health Perspect 126:065001
Chen, Po-Yen; Zhang, Mengke; Liu, Muchun et al. (2018) Ultrastretchable Graphene-Based Molecular Barriers for Chemical Protection, Detection, and Actuation. ACS Nano 12:234-244
Wilson, Shelby; Dere, Edward; Klein, David et al. (2018) Localization of dimethylated histone three lysine four in the Rattus norvegicus sperm genome. Biol Reprod 99:266-268
Kane, Agnes B; Hurt, Robert H; Gao, Huajian (2018) The asbestos-carbon nanotube analogy: An update. Toxicol Appl Pharmacol 361:68-80

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