The major objective of this new Research Component is to selectively breed replicate mouse lines for high and low alcohol preference (HAP and LAP) starting from the HS/Ibg heterogeneous stock. This breeding program will also include replicate control lines. Since the mouse genome is much better delineated than that of the rat, we hope that in the future the HAP and LAP mouse lines can facilitate the localization of genes relevant in determining alcohol drinking behavior. For the next five years, efforts will be devoted to the following: (1) selective breeding of the replicate HAP, LAP and control lines; (2) calculating the realized heritability for the divergent selection; (3) characterizing the alcohol drinking behaviors of the HAP and LAP replicate lines, e.g. pattern of alcohol drinking bouts, blood ethanol concentrations attainable with each drinking bout, elasticity of alcohol drinking when diet is manipulated, and development of conditioned taste aversion; and (4) determining the relationship of high and low alcohol preference to other alcohol-related traits, e.g. alcohol elimination rates, hepatic ADH and ALDH activities, stimulation of spontaneous motor activity with low-dose ethanol, initial sensitivity and rapid development of acute tolerance in response to high- dose ethanol, operant responding (e.g. head-poke or bar-pressing) to obtain alcohol, and brain contents of serotonin (5HT) and dopamine (DA) and their metabolites. Most of the methods to be used are already established in our Alcohol Research Center. Few new methods have to be adopted from other workers in the field and they should not constitute major obstacles. consultation help from Dr. Donald P. Doolittle (The Jackson Laboratory) and Dr. R. Thomas Gentry (VAMC, Bronx, NY) will facilitate many aspects of this new Research Component.

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Specialized Center (P50)
Project #
2P50AA007611-06
Application #
3767697
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Indiana University Bloomington
Department
Type
DUNS #
006046700
City
Bloomington
State
IN
Country
United States
Zip Code
47401
McClintick, Jeanette N; McBride, William J; Bell, Richard L et al. (2018) Gene expression changes in the ventral hippocampus and medial prefrontal cortex of adolescent alcohol-preferring (P) rats following binge-like alcohol drinking. Alcohol 68:37-47
Houck, Christa A; Grahame, Nicholas J (2018) Acute drug effects on habitual and non-habitual responding in crossed high alcohol preferring mice. Psychopharmacology (Berl) 235:2167-2175
Ding, Zheng-Ming; Ingraham, Cynthia M; Hauser, Sheketha R et al. (2017) Reduced Levels of mGlu2 Receptors within the Prelimbic Cortex Are Not Associated with Elevated Glutamate Transmission or High Alcohol Drinking. Alcohol Clin Exp Res 41:1896-1906
Linsenbardt, David N; Smoker, Michael P; Janetsian-Fritz, Sarine S et al. (2017) Impulsivity in rodents with a genetic predisposition for excessive alcohol consumption is associated with a lack of a prospective strategy. Cogn Affect Behav Neurosci 17:235-251
Bell, Richard L; Hauser, Sheketha R; Liang, Tiebing et al. (2017) Rat animal models for screening medications to treat alcohol use disorders. Neuropharmacology 122:201-243
Öztürk, Nail Can; Resendiz, Marisol; Öztürk, Hakan et al. (2017) DNA Methylation program in normal and alcohol-induced thinning cortex. Alcohol 60:135-147
Luczak, Susan E; Wall, Tamara L (2016) Gambling problems and comorbidity with alcohol use disorders in Chinese-, Korean-, and White-American college students. Am J Addict 25:195-202
Ding, Zheng-Ming; Ingraham, Cynthia M; Rodd, Zachary A et al. (2016) Alcohol drinking increases the dopamine-stimulating effects of ethanol and reduces D2 auto-receptor and group II metabotropic glutamate receptor function within the posterior ventral tegmental area of alcohol preferring (P) rats. Neuropharmacology 109:41-48
Kasten, C R; Frazee, A M; Boehm 2nd, S L (2016) Developing a model of limited-access nicotine consumption in C57Bl/6J mice. Pharmacol Biochem Behav 148:28-37
Zhou, Feng C; Resendiz, Marisol; Lo, Chiao-Ling et al. (2016) Cell-Wide DNA De-Methylation and Re-Methylation of Purkinje Neurons in the Developing Cerebellum. PLoS One 11:e0162063

Showing the most recent 10 out of 253 publications