The overall goals of this proposal are to develop a center which will foster research concerning the role of ethanol on: (1) cellular immune function in animal models, (2) immunosuppression and development of acquired immunodeficiency diseases including retrovirus infection, and (3) mechanisms of liver toxicity via immunomodulation. Our approaches involve a multidisciplinary, basic science-oriented, research effort. It will develop an alcohol research center and include: 1) The relationships of alcohol use, retrovirus infection, immunomodulation and AIDS development to: - determine alcohol's effects on death rates - investigate in depth alcohol's actions on immune functions in mice with various levels of alcohol intake. - study in detail the immunological changes due to alcohol as a cofactor during retroviral infection on resistance to other pathogens. - determine ways to stimulate ethanol-suppressed host defenses and disease resistance during retroviral infection. 2) The use of well-defined animal model systems to: - determine interactions between alcohol use, nutritional stresses, immunoarchitecture changes and the resulting changes in resistance to murine retrovirus. - investigate the long-term effects of alcohol to modulate cellular immune functions as they correlate with enhancement of chemically- induced liver cancer in rats. - determine the role of toxin and alcohol-altered Kupffer cell functions in liver damage in rats. -study alcohol's effects on sinusoidal permeability, tissue oxygenation, cell migration in lymph. - measure and study host defense function of macrophages and Kupffer cells in vivo including in vivo microscopy as modified by ethanol and retroviral disease.

National Institute of Health (NIH)
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Specialized Center (P50)
Project #
Application #
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Arizona
Schools of Medicine
United States
Zip Code
Sørensen, Karen Kristine; Simon-Santamaria, Jaione; McCuskey, Robert S et al. (2015) Liver Sinusoidal Endothelial Cells. Compr Physiol 5:1751-74
Urbaschek, R; McCuskey, R S; Rudi, V et al. (2001) Endotoxin, endotoxin-neutralizing-capacity, sCD14, sICAM-1, and cytokines in patients with various degrees of alcoholic liver disease. Alcohol Clin Exp Res 25:261-8
Colombo, L L; Lopez, M C; Chen, G et al. (2001) In vitro response of v-Ha-ras transgenic mouse lymphocytes after in vivo treatment with cocaine. Immunopharmacol Immunotoxicol 23:607-16
Colombo, L L; Lopez, M C; Chen, G et al. (2001) In vitro response of v-Ha-ras transgenic mouse lymphocytes after in vivo treatment with alcohol. Immunopharmacol Immunotoxicol 23:597-606
Colombo, L L; Stazzone, A; Chen, G J et al. (2000) Behavioral and immune changes in v-Ha-ras transgenic mice. Immunopharmacol Immunotoxicol 22:339-56
Nishida, J; McDonnell, D; McCuskey, M K et al. (1999) In vivo and electron microscopic observations of the responses of the hepatic sinusoid to interleukin-1. Bull Tokyo Dent Coll 40:139-48
Colombo, L L; Lopez, M C; Chen, G J et al. (1999) Effect of short-term cocaine administration on the immune system of young and old C57BL/6 female mice. Immunopharmacol Immunotoxicol 21:755-69
Watson, R R; Solkoff, D; Wang, J Y et al. (1998) Detection of ethanol consumption by ELISA assay measurement of acetaldehyde adducts in murine hair. Alcohol 16:279-84
Wang, J Y; Liang, B; Watson, R R (1997) Alcohol consumption alters cytokine release during murine AIDS. Alcohol 14:155-9
Hoglen, N C; Abril, E A; Sauer, J M et al. (1997) Modulation of Kupffer cell and peripheral blood monocyte activity by in vivo treatment of rats with all-trans-retinol. Liver 17:157-65

Showing the most recent 10 out of 113 publications