The imaging core has two components that provide major support to the center investigators. The Microscopy Component provides the following: 1) high resolution 3- dimensional imaging of parameter-sensitive fluorophores in living cells and tissues by laser scanning confocal microscopy to monitor ions, electrical potentials, oxygen radical generation, pyradine nucleotide reduction, mitochondrial and plasmalemmal membrane permeability, and cell viability (apoptosis and necrosis); 2) intravitral microscopy using a """"""""real time"""""""" confocal microscope to monitor microcirculation, leukocyte margination and gene expression in specific cell populations of the livers of anaesthetized rats; 3) high resolution confocal imaging of tissue sections for immunocytochemistry, fluorescence in situ hybridization and green fluorescent protein; 4) a digital darkroom facility for digitizing images, analyzing and labeling these images, and printing the processed images as photographic quality prints and slides for use in publications, reports and oral presentation; and 5) a fluorescent plate reader for measuring cell viability and intracellular ions in cultured cells grown on multi-well plates. The Pathology Component provides consultation, training and services in basic morphological and immunohistochemical techniques for preparation of frozen and paraffin sections of histology, immunocytochemistry, and in situ hybridization. The Pathology Component routinely handles H&E, PAS, eosin, and trichrome staining of brain, liver and other tissue and provides immunocytochemical staining for glial fibrillary protein (GFAP), myelin basic protein (MBP), lectin binding and other cellular components of interest.

National Institute of Health (NIH)
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Specialized Center (P50)
Project #
Application #
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of North Carolina Chapel Hill
Chapel Hill
United States
Zip Code
Jaramillo, Anel A; Randall, Patrick A; Stewart, Spencer et al. (2018) Functional role for cortical-striatal circuitry in modulating alcohol self-administration. Neuropharmacology 130:42-53
Bohnsack, John Peyton; Hughes, Benjamin A; O'Buckley, Todd K et al. (2018) Histone deacetylases mediate GABAA receptor expression, physiology, and behavioral maladaptations in rat models of alcohol dependence. Neuropsychopharmacology 43:1518-1529
Coleman Jr, Leon G; Zou, Jian; Qin, Liya et al. (2018) HMGB1/IL-1? complexes regulate neuroimmune responses in alcoholism. Brain Behav Immun 72:61-77
Coleman Jr, Leon G; Crews, Fulton T (2018) Innate Immune Signaling and Alcohol Use Disorders. Handb Exp Pharmacol 248:369-396
Vetreno, Ryan P; Patel, Yesha; Patel, Urvi et al. (2017) Adolescent intermittent ethanol reduces serotonin expression in the adult raphe nucleus and upregulates innate immune expression that is prevented by exercise. Brain Behav Immun 60:333-345
Vetreno, Ryan P; Yaxley, Richard; Paniagua, Beatriz et al. (2017) Adult rat cortical thickness changes across age and following adolescent intermittent ethanol treatment. Addict Biol 22:712-723
Crews, Fulton T; Walter, T Jordan; Coleman Jr, Leon G et al. (2017) Toll-like receptor signaling and stages of addiction. Psychopharmacology (Berl) 234:1483-1498
Coleman Jr, Leon G; Zou, Jian; Crews, Fulton T (2017) Microglial-derived miRNA let-7 and HMGB1 contribute to ethanol-induced neurotoxicity via TLR7. J Neuroinflammation 14:22
Radke, Anna K; Jury, Nicholas J; Kocharian, Adrina et al. (2017) Chronic EtOH effects on putative measures of compulsive behavior in mice. Addict Biol 22:423-434
Salling, Michael C; Hodge, Christopher J; Psilos, Kelly E et al. (2017) Cue-induced reinstatement of alcohol-seeking behavior is associated with increased CaMKII T286 phosphorylation in the reward pathway of mice. Pharmacol Biochem Behav 163:20-29

Showing the most recent 10 out of 126 publications