Pilot Study - Innervation of Basal Forebrain Cholinergic Neurons

Martin, Lee J.

Abstract

In order to understand some of the functions of neurons in the basal forebrain in magnocellular complex (BFMC), it is essential to identify the sources and transmitter specificities of afferents to this system. Using axonal tracing and transmitter immunocytochemistry (ICC), Pilot 4 is designed to characterize, at the light and ultrastructural levels, neural systems that innervate BFMC cholinergic neurons in rodents. The anterograde tracer Phaseolus vulagaris leukoagglutinin (PHA-L) will be iontophoresed into regions known to be labeled following injections of retrograde tracer into subdivisions of the BFMC, and dual-color ICC will be employed to identify specific interactions between PHA-L-labeled boutons and cholinergic neurons. Monoaminergic, cholinergic, gamma- aminobutyric acid (GABA)ergic, and peptidergic transmitter-specific interactions with cholinergic neurons of the BFMC will be identified at the electron-microscopic level using double-label ICC> These studies will provide new insights concerning afferents that modulate cholinergic BFMC neurons in rodents and are essential for similar investigations of these afferents in primates.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute on Aging (NIA)
• Type: Specialized Center (P50)
• Project #: 5P50AG005146-08
• Application #: 3809234
• Support Year: 8
• Fiscal Year: 1990

Institution

• Name: Johns Hopkins University
• DUNS #: 045911138
• City: Baltimore
• State: MD
• Country: United States
• Zip Code: 21218

Publications

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Nicolas, Aude (see original citation for additional authors) (2018) Genome-wide Analyses Identify KIF5A as a Novel ALS Gene. Neuron 97:1268-1283.e6

Wong, Dean F; Comley, Robert A; Kuwabara, Hiroto et al. (2018) Characterization of 3 Novel Tau Radiopharmaceuticals, 11C-RO-963, 11C-RO-643, and 18F-RO-948, in Healthy Controls and in Alzheimer Subjects. J Nucl Med 59:1869-1876

Kirson, Noam Y; Scott Andrews, J; Desai, Urvi et al. (2018) Patient Characteristics and Outcomes Associated with Receiving an Earlier Versus Later Diagnosis of Probable Alzheimer's Disease. J Alzheimers Dis 61:295-307

Varma, Vijay R; Oommen, Anup M; Varma, Sudhir et al. (2018) Brain and blood metabolite signatures of pathology and progression in Alzheimer disease: A targeted metabolomics study. PLoS Med 15:e1002482

Zhou, Zilu; Wang, Weixin; Wang, Li-San et al. (2018) Integrative DNA copy number detection and genotyping from sequencing and array-based platforms. Bioinformatics 34:2349-2355

Soldan, Anja; Pettigrew, Corinne; Albert, Marilyn (2018) Evaluating Cognitive Reserve Through the Prism of Preclinical Alzheimer Disease. Psychiatr Clin North Am 41:65-77

Guerreiro, Rita; Ross, Owen A; Kun-Rodrigues, Celia et al. (2018) Investigating the genetic architecture of dementia with Lewy bodies: a two-stage genome-wide association study. Lancet Neurol 17:64-74

Bermudez, Camilo; Plassard, Andrew J; Davis, Taylor L et al. (2018) Learning Implicit Brain MRI Manifolds with Deep Learning. Proc SPIE Int Soc Opt Eng 10574: