Abstract

Several types of botanical products containing isoflavones are being marketed as an alternative to estrogen replacement therapy to prevent osteoporosis in postmenopausal women. Estrogen suppresses bone resorption and may increase calcium absorption. Efficacy of isoflavones on bone health has mixed results, which may depend on the dose and interaction of isoflavones and the ability of individuals to produce equol.
Specific Aim 1 is to determine the dose response of effect of genistein and the interaction with other soy isoflavones on calcium absorption and bone resorption in postmenopausal women who are equol vs. nonequolproducers. We will use 41Ca, a long-lived isotope, to label the skeleton and an Accelerator Mass Spectrometry (AMS) to monitor perturbations by diet. This innovative approach to study bone resorption is a direct measure of bone resorption in contrast to biochemical markers of bone turnover and traditional calcium kinetics, rapid compared to studying changes in bone density, and not invasive as is bone histomorphometry. We are able to systematically determine the effect of various types and dosages of isoflavone-containing botanical products sequentially in the same postmenopausal women for their effectiveness in suppressing bone resorption by measuring changes in 41Ca excretion in the urine. Preliminary evidence indicates that kudzu root, marketed as an estrogen replacement, reduces bone loss, which may relate to an unusual isoflavone, puerarin.
Specific Aim 2 is to measure the dose response effect of genistein, kudzu, and equol on bone and bone quality in an ovariectomized rat model.
Specific Aim 3 is to study metabolism of isoflavones in soybeans and kudzu. We have developed the ability to prepare C-14 and C-13 labeled plant tissue culture. By gavaging purified or crude fractions to rats with implanted microdialysis probes, we can follow the metabolic fate of bioactive compounds throughout the body. Minute quantities can be traced with AMS and microNMR. ? ?

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Complementary & Alternative Medicine (NCCAM)
Type
Specialized Center (P50)
Project #
5P50AT000477-09
Application #
7405486
Study Section
Special Emphasis Panel (ZAT1-DB (17))
Program Officer
Sorkin, Barbara C
Project Start
2000-09-30
Project End
2010-03-31
Budget Start
2008-04-01
Budget End
2009-03-31
Support Year
9
Fiscal Year
2008
Total Cost
$1,225,000
Indirect Cost

  Institution

Name
Purdue University
Department
Nutrition
Type
Other Domestic Higher Education
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907

  Publications

Barnes, Stephen; Quinlan, Roy A (2017) Small molecules, both dietary and endogenous, influence the onset of lens cataracts. Exp Eye Res 156:87-94
Jacobi, Jennifer L; Yang, Bo; Li, Xu et al. (2016) Impacts on Sirtuin Function and Bioavailability of the Dietary Bioactive Compound Dihydrocoumarin. PLoS One 11:e0149207
Jackson, George S; Muzikar, Paul; Goehring, Brent (2015) A Bayesian approach to an interlaboratory comparison. Chemometr Intell Lab Syst 141:94-99
Pawlowski, Jessica W; Martin, Berdine R; McCabe, George P et al. (2015) Impact of equol-producing capacity and soy-isoflavone profiles of supplements on bone calcium retention in postmenopausal women: a randomized crossover trial. Am J Clin Nutr 102:695-703
Roysommuti, Sanya; Kritsongsakchai, Angkana; Wyss, J Michael (2015) The Effect of Perinatal Taurine on Adult Renal Function Does Not Appear to Be Mediated by Taurine's Inhibition of the Renin-Angiotensin System. Adv Exp Med Biol 803:665-77
Legette, LeeCole L; Prasain, Jeevan; King, Jennifer et al. (2014) Pharmacokinetics of equol, a soy isoflavone metabolite, changes with the form of equol (dietary versus intestinal production) in ovariectomized rats. J Agric Food Chem 62:1294-300
Theis, Whitney S; Andringa, Kelly K; Millender-Swain, Telisha et al. (2014) Ozone inhalation modifies the rat liver proteome. Redox Biol 2:52-60
Roysommuti, Sanya; Wyss, J Michael (2014) Perinatal taurine exposure affects adult arterial pressure control. Amino Acids 46:57-72
Nakatsu, Cindy H; Armstrong, Arthur; Clavijo, Andrea P et al. (2014) Fecal bacterial community changes associated with isoflavone metabolites in postmenopausal women after soy bar consumption. PLoS One 9:e108924
Abdul Roda, Mojtaba; Sadik, Mariam; Gaggar, Amit et al. (2014) Targeting prolyl endopeptidase with valproic acid as a potential modulator of neutrophilic inflammation. PLoS One 9:e97594

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