Abstract

This proposal for a Genomics Core Laboratory (GCL) within the UPCI Lung Cancer SPORE program represents a coordinated, collaborative effort on the part of the General Clinical Research Center (GCRC) Pharmacogenetics Core Laboratory (PCL) in the Center for Clinical Pharmacology and the TagMan Facility in the University of Pittsburgh Cancer Institute (UPCI) at the University of Pittsburgh. In addition, the GCL facility will utilize expertise and instrumentation available in the Center for Genomics Sciences (CGS) and the University of Pittsburgh DNA microarray facility (PittArray) for DNA sequencing and cDNA microarray analysis respectively This GCL facility will provide a sophisticated centralized, efficient, low cost mechanism for application of a panel of genotyping, mRNA expression and sequencing endpoint measurements and for development and validation of novel endpoints. These capabilities will be provided either as a full service (genotyping) or as a training/consultation service where GCL staff provide the training, expertise, and equipment for researchers to carry out their own experiments (quantitative polymerase chain reaction (QPCR), sequencing and microarrays). To this end, a technically innovative and well-organized core laboratory is proposed. The GCL will also be involved in new assay development including a high-throughput genotyping approach and improved mRNA quantitation assays. The centralized natured of this facility will permit stringent quality control and economic utilization of laboratory resources. Unique aspects of this core laboratory feature a clear line of command, dedicated leadership management, technical versatility of experienced laboratory personnel, a centralized database and sample tracking procedures, provisions for technical development and addition of related genetic endpoints as information becomes available in the public domain. Furthermore, the GCL is committed to participating in a collaborative interaction with the other components of the UPCI Lung Cancer SPORE.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center (P50)
Project #
1P50CA090440-01
Application #
6480545
Study Section
Special Emphasis Panel (ZCA1)
Project Start
2001-06-01
Project End
2006-04-30
Budget Start
Budget End
Support Year
1
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
University of Pittsburgh
Department
Type
DUNS #
053785812
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213

  Publications

Leng, Shuguang; Diergaarde, Brenda; Picchi, Maria A et al. (2018) Gene Promoter Hypermethylation Detected in Sputum Predicts FEV1 Decline and All-Cause Mortality in Smokers. Am J Respir Crit Care Med 198:187-196
Rothenberger, Natalie J; Somasundaram, Ashwin; Stabile, Laura P (2018) The Role of the Estrogen Pathway in the Tumor Microenvironment. Int J Mol Sci 19:
Yochum, Zachary A; Cades, Jessica; Wang, Hailun et al. (2018) Targeting the EMT transcription factor TWIST1 overcomes resistance to EGFR inhibitors in EGFR-mutant non-small-cell lung cancer. Oncogene :
Stabile, Laura P; Farooqui, Mariya; Kanterewicz, Beatriz et al. (2018) Preclinical Evidence for Combined Use of Aromatase Inhibitors and NSAIDs as Preventive Agents of Tobacco-Induced Lung Cancer. J Thorac Oncol 13:399-412
Volonte, Daniela; Vyas, Avani R; Chen, Chen et al. (2018) Caveolin-1 promotes the tumor suppressor properties of oncogene-induced cellular senescence. J Biol Chem 293:1794-1809
Hopkins, Kathleen G; Ferson, Peter F; Shende, Manisha R et al. (2017) Prospective study of quality of life after lung cancer resection. Ann Transl Med 5:204
Vendetti, Frank P; Leibowitz, Brian J; Barnes, Jennifer et al. (2017) Pharmacologic ATM but not ATR kinase inhibition abrogates p21-dependent G1 arrest and promotes gastrointestinal syndrome after total body irradiation. Sci Rep 7:41892
Tarhini, Ahmad A; Rafique, Imran; Floros, Theofanis et al. (2017) Phase 1/2 study of rilotumumab (AMG 102), a hepatocyte growth factor inhibitor, and erlotinib in patients with advanced non-small cell lung cancer. Cancer 123:2936-2944
Sun, Fan; Xiao, Gutian; Qu, Zhaoxia (2017) Isolation of Murine Alveolar Type II Epithelial Cells. Bio Protoc 7:
Dandachi, Nadine; Kelly, Neil J; Wood, John P et al. (2017) Macrophage Elastase Induces TRAIL-mediated Tumor Cell Death through Its Carboxy-Terminal Domain. Am J Respir Crit Care Med 196:353-363

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