The specific aims of this proposal are 1)To collect, process, bank and distribute human colorectal neoplasmsand matched normal tissue samples to investigators in the Vanderbilt Gl SPORE and other Gl SPORES;2)To collect and bank portions of polyps and biopsies of grossly normal colorectal mucosa to facilitateresearch in adenoma recurrence; 3)To perform quality control to ensure that the relevant tissue is supplied tothe researcher and that tissues are suitable for the planned research (not necrotic or involved byunsuspected disease processes); 4)To protect patient confidentiality through use of an explicit consent formthat specifically addresses use of extraneous tissue for research purposes and through de-identification ofspecimens; 5)To work with the Biostatistics/Bioinformatics Core to establish an informatics strategy fornetworking of requests, specimen tracking, extraction of de-identified data relating to specimens of interestand linkage to research data. 6) To provide laser capture microdissection services to the Gl SPOREinvestigators; 7)To provide tissue microarray services to the Gl SPORE investigators and provide TMA slidesto investigators at other institutions (such as the H. Lee Moffitt Cancer Center) and other Gl SPOREs; 8)Toprovide expertise in evaluation of histopathology of mouse models of colorectal neoplasia and correlationwith human disease; 10)To provide expertise in developing, performing and evaluating immunohistochemicalstains for Gl SPORE investigators. The Gl SPORE Tissue Core at VUMC has partnered with othermechanisms for tissue collection at VUMC under Dr. Washington's direction: the Human Tissue Acquisitionand Pathology Shared Resource of the Vanderbilt-lngram Comprehensive Cancer Center, the TissueMorphology SubCore for the Digestive Disease Research Center and the VUMC-led Western Division of theCooperative Human Tissue Network. Mechanisms and standard operating procedures for collection ofhuman Gl tissue, quality assurance and immunohistochemistry have been used to support all five SPOREprojects in the previous funding period. By partnering with other tissue and histology resources at VUMC, theGl SPORE Tissue Core is able to provide high quality services to SPORE investigators in a highly costeffective manner.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center (P50)
Project #
2P50CA095103-06
Application #
7245710
Study Section
Special Emphasis Panel (ZCA1-GRB-I (J1))
Project Start
2007-05-01
Project End
2012-04-30
Budget Start
2007-07-25
Budget End
2008-04-30
Support Year
6
Fiscal Year
2007
Total Cost
$144,795
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
Davenport, James R; Su, Timothy; Zhao, Zhiguo et al. (2018) Modifiable lifestyle factors associated with risk of sessile serrated polyps, conventional adenomas and hyperplastic polyps. Gut 67:456-465
Liu, Qi; Herring, Charles A; Sheng, Quanhu et al. (2018) Quantitative assessment of cell population diversity in single-cell landscapes. PLoS Biol 16:e2006687
Means, Anna L; Freeman, Tanner J; Zhu, Jing et al. (2018) Epithelial Smad4 Deletion Up-Regulates Inflammation and Promotes Inflammation-Associated Cancer. Cell Mol Gastroenterol Hepatol 6:257-276
Weiss, Vivian L; Kiernan, Colleen; Wright, Jesse et al. (2018) Fine-Needle Aspiration-Based Grading of Pancreatic Neuroendocrine Neoplasms Using Ki-67: Is Accurate WHO Grading Possible on Cytologic Material? J Am Soc Cytopathol 7:154-459
Roberts, Jordan; Gonzalez, Raul S; Revetta, Frank et al. (2018) Mesenteric tumour deposits arising from small-intestine neuroendocrine tumours are frequently associated with fibrosis and IgG4-expressing plasma cells. Histopathology 73:795-800
Gibson, William E; Gonzalez, Raul S; Cates, Justin M M et al. (2018) Hepatic micrometastases are associated with poor prognosis in patients with liver metastases from neuroendocrine tumors of the digestive tract. Hum Pathol 79:109-115
Fenix, Aidan M; Neininger, Abigail C; Taneja, Nilay et al. (2018) Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes. Elife 7:
Wang, Jing; Zhao, Yue; Zhou, Xiaofan et al. (2018) Nascent RNA sequencing analysis provides insights into enhancer-mediated gene regulation. BMC Genomics 19:633
Burns, Michael C; Howes, Jennifer E; Sun, Qi et al. (2018) High-throughput screening identifies small molecules that bind to the RAS:SOS:RAS complex and perturb RAS signaling. Anal Biochem 548:44-52
Herring, Charles A; Banerjee, Amrita; McKinley, Eliot T et al. (2018) Unsupervised Trajectory Analysis of Single-Cell RNA-Seq and Imaging Data Reveals Alternative Tuft Cell Origins in the Gut. Cell Syst 6:37-51.e9

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