The purpose of this shared resource is to collect and distribute clinically annotated human biospecimens related to cervical cancer and HPV disease, and to provide pathologic and immunologic expertise and support for tissue-based analyses for investigators in the Johns Hopkins/University of Alabama/University of Colorado/Mount Sinai Cervical Cancer SPORE. The tissue core has been in existence since 1998, and has expanded with the support of the Cervical Cancer SPORE. To date, we have banked frozen tissue samples from 810 benign gynecologic tissues, 2,220 tumor resections, and 178 resections of preinvasive cervical HPV lesions. Longitudinally obtained exfoliated cell and peripheral blood specimens from 245 subjects enrolled in our CIN2/3 protocols have also been obtained, as well as one-time subject-matched peripheral blood samples for benign tissues (206), and from 632 tumor samples. Formalin fixed blocks are available for virtually all of these specimens. In addition, this shared resource has also banked specimens from patients enrolled in therapeutic vaccination protocols for HPV-associated squamous cancers of the head and neck. All biospecimens are harvested and banked in accordance with the National Cancer Institute?s Best Practice Guidelines for Biorepositories. The core has been expanded to include expert pathologic and immunologic consultation to investigators, including guidance for quantitative digital image analyses-guided molecular studies of tissues. Specimens will be collected under the supervision of pathologists with expertise in gynecologic pathology, in close collaboration with clinical colleagues in these areas. Clinical information for subjects enrolled in our clinical protocols is entered into a password-protected web-based tracking system. This internal web-based system follows the recommendations of the National Research Council, and includes user authentication, encryption, audit trails, and disaster recovery. A review mechanism is in place for prioritization of distribution of requested resources to investigators within and external to the Johns Hopkins Cervical Cancer SPORE. Biosamples have been shared with collaborators at academic institutions across the country.

Public Health Relevance

Despite many prevention strategies, HPV-associated disease remains common, not only in the female lower genital tract, but also in anatomic sites for which no screening algorithms have been validated. This core resource will support projects of this application related to the diagnosis and treatment of HPV neoplasia with high quality tissues and biosamples as well as to provide pathologic and immunologic expertise.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Specialized Center (P50)
Project #
Application #
Study Section
Special Emphasis Panel (ZCA1-RPRB-C (M1))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Johns Hopkins University
United States
Zip Code
Boitano, Teresa K L; Smith, Haller J; Rushton, Tullia et al. (2018) Impact of enhanced recovery after surgery (ERAS) protocol on gastrointestinal function in gynecologic oncology patients undergoing laparotomy. Gynecol Oncol 151:282-286
Anchoori, Ravi K; Jiang, Rosie; Peng, Shiwen et al. (2018) Covalent Rpn13-Binding Inhibitors for the Treatment of Ovarian Cancer. ACS Omega 3:11917-11929
Ooki, Akira; Dinalankara, Wikum; Marchionni, Luigi et al. (2018) Epigenetically regulated PAX6 drives cancer cells toward a stem-like state via GLI-SOX2 signaling axis in lung adenocarcinoma. Oncogene 37:5967-5981
Ahn, Julie; Bishop, Justin A; Roden, Richard B S et al. (2018) The PD-1 and PD-L1 pathway in recurrent respiratory papillomatosis. Laryngoscope 128:E27-E32
Silver, Michelle I; Rositch, Anne F; Phelan-Emrick, Darcy F et al. (2018) Uptake of HPV testing and extended cervical cancer screening intervals following cytology alone and Pap/HPV cotesting in women aged 30-65 years. Cancer Causes Control 29:43-50
Yang, J-Ming; Bhattacharya, Sayak; West-Foyle, Hoku et al. (2018) Integrating chemical and mechanical signals through dynamic coupling between cellular protrusions and pulsed ERK activation. Nat Commun 9:4673
Xing, Deyin; Zheng, Gang; Schoolmeester, John Kenneth et al. (2018) Next-generation Sequencing Reveals Recurrent Somatic Mutations in Small Cell Neuroendocrine Carcinoma of the Uterine Cervix. Am J Surg Pathol 42:750-760
Qiu, Jin; Peng, Shiwen; Ma, Ying et al. (2018) Epithelial boost enhances antigen expression by vaccinia virus for the generation of potent CD8+ T cell-mediated antitumor immunity following DNA priming vaccination. Virology 525:205-215
Ooki, Akira; Begum, Asma; Marchionni, Luigi et al. (2018) Arsenic promotes the COX2/PGE2-SOX2 axis to increase the malignant stemness properties of urothelial cells. Int J Cancer 143:113-126
Leath 3rd, Charles A; Monk, Bradley J (2018) Twenty-first century cervical cancer management: A historical perspective of the gynecologic oncology group/NRG oncology over the past twenty years. Gynecol Oncol 150:391-397

Showing the most recent 10 out of 291 publications