Abstract

The Image Core facility will provide three categories of services to the Drug Abuse Research Center: 1) imaging of labelled cells and whole brains at high resolution and detail. 2) Live cell imaging and measurement capabilities. 3) Image processing and enhancement capabilities for better separation of regions of interest from background. The main purpose of the core is to provide investigators with training regions of interest from background. The main purpose of the core is to provide investigators with training and with appropriate imaging devices to see where markers have localized, look for differences in morphologies and colocalization, and record movement of calcium. The core will also train investigators ib software to measure areas and volumes, and to enhance images. This core also posts training materials on its web site for other researchers, updating as new information arises.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Specialized Center (P50)
Project #
5P50DA011806-04
Application #
6469774
Study Section
Project Start
2001-07-01
Project End
2002-06-30
Budget Start
Budget End
Support Year
4
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
University of Minnesota Twin Cities
Department
Type
DUNS #
168559177
City
Minneapolis
State
MN
Country
United States
Zip Code
55455

  Publications

Hwang, Cheol Kyu; Wagley, Yadav; Law, Ping-Yee et al. (2017) Phosphorylation of poly(rC) binding protein 1 (PCBP1) contributes to stabilization of mu opioid receptor (MOR) mRNA via interaction with AU-rich element RNA-binding protein 1 (AUF1) and poly A binding protein (PABP). Gene 598:113-130
Kibaly, Cherkaouia; Lin, Hong-Yiou; Loh, Horace H et al. (2017) Spinal or supraspinal phosphorylation deficiency at the MOR C-terminus does not affect morphine tolerance in vivo. Pharmacol Res 119:153-168
Kibaly, Cherkaouia; Kam, Angel Y F; Loh, Horace H et al. (2016) Naltrexone Facilitates Learning and Delays Extinction by Increasing AMPA Receptor Phosphorylation and Membrane Insertion. Biol Psychiatry 79:906-16
Meng, Jingjing; Roy, Sabita (2016) Study of Epithelium Barrier Functions by Real-time TER Measurement. Bio Protoc 6:
Banerjee, S; Sindberg, G; Wang, F et al. (2016) Opioid-induced gut microbial disruption and bile dysregulation leads to gut barrier compromise and sustained systemic inflammation. Mucosal Immunol 9:1418-1428
Wang, Yan; Wang, Yan-Xia; Liu, Ting et al. (2015) ?-Opioid receptor attenuates A? oligomers-induced neurotoxicity through mTOR signaling. CNS Neurosci Ther 21:8-14
Meng, Jingjing; Banerjee, Santanu; Li, Dan et al. (2015) Opioid Exacerbation of Gram-positive sepsis, induced by Gut Microbial Modulation, is Rescued by IL-17A Neutralization. Sci Rep 5:10918
Kotecki, Lydia; Hearing, Matthew; McCall, Nora M et al. (2015) GIRK Channels Modulate Opioid-Induced Motor Activity in a Cell Type- and Subunit-Dependent Manner. J Neurosci 35:7131-42
Hwang, Cheol Kyu; Wagley, Yadav; Law, Ping-Yee et al. (2015) Analysis of epigenetic mechanisms regulating opioid receptor gene transcription. Methods Mol Biol 1230:39-51
Wang, Yan; Ge, Yan-Hui; Wang, Yan-Xia et al. (2015) Modulation of mTOR Activity by ?-Opioid Receptor is Dependent upon the Association of Receptor and FK506-Binding Protein 12. CNS Neurosci Ther 21:591-8

Showing the most recent 10 out of 308 publications