Relapse to drug use following periods of abstinence is a significant impediment in the long-term treatment of cocaine dependence. Environmental stimuli or contexts previously associated with drug use or priming doses of the abused drug can initiate relapse to compulsive drug-seeking and drug-taking behaviors. The Animal Core B will provide all of the experimental subjects for every project in the Neurobiology of Addiction Research Center (NARC) that involves the use of the self-administration and relapse model in rats. Rats will be catheterized and trained to self-administer intravenous (i.v.) cocaine using standard schedules of drug reinforcement. After a stable period of self-administration, lever pressing for drug will be extinguished to criterion, or animals may undergo abstinence. Subjects will then be directed towards individual NARC projects for brain tissue extraction, electrophysiology, or assessment of behavioral responding to systemic or site directed manipulations in accordance with the specific aims of each NARC project.
The aims of Animal Core B can be summarized as: 1. Provide experimental subjects to all projects in the NARC that are consistent and satisfy specific criteria with regards to experience in drug self-administration and in the degree of withdrawal, extinction, and/or abstinence. The Animal Core will insure that subjects will be handled and treated according to all approved animal use procedures and, in conjunction with vivarium staff, maintain appropriate animal husbandry. 2. Efficiently manage the distribution of animals to the different projects to insure that statistically relevant group sizes and group replications are made available to each project in a timely manner. 3. Maintain a staff of well-trained technicians to efficiently generate experimental subjects using a balanced group design, as well as provide technical assistance to NARC Projects that will involve further behavioral evaluation of drug-seeking behavior. 4. Serve as a NARC training center for junior investigators who desire training in self-administration, jugular catheterization, operant conditioning methods, and other related experimental procedures. The NARC Animal Core will serve as the common foundation for all NARC projects. The results from integrated studies through the Animal Core will hopefully lead to useful pharmacotherapeutic agents for the treatment of cocaine abuse and dependence.

National Institute of Health (NIH)
National Institute on Drug Abuse (NIDA)
Specialized Center (P50)
Project #
Application #
Study Section
Special Emphasis Panel (ZDA1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Medical University of South Carolina
United States
Zip Code
Hanlon, Colleen A; Dowdle, Logan T; Gibson, Nicole B et al. (2018) Cortical substrates of cue-reactivity in multiple substance dependent populations: transdiagnostic relevance of the medial prefrontal cortex. Transl Psychiatry 8:186
Roberts-Wolfe, Douglas; Bobadilla, Ana-Clara; Heinsbroek, Jasper A et al. (2018) Drug Refraining and Seeking Potentiate Synapses on Distinct Populations of Accumbens Medium Spiny Neurons. J Neurosci 38:7100-7107
Spencer, Sade; Neuhofer, Daniela; Chioma, Vivian C et al. (2018) A Model of ?9-Tetrahydrocannabinol Self-administration and Reinstatement That Alters Synaptic Plasticity in Nucleus Accumbens. Biol Psychiatry 84:601-610
Parrilla-Carrero, Jeffrey; Buchta, William C; Goswamee, Priyodarshan et al. (2018) Restoration of Kv7 Channel-Mediated Inhibition Reduces Cued-Reinstatement of Cocaine Seeking. J Neurosci 38:4212-4229
Kearney-Ramos, Tonisha E; Lench, Daniel H; Hoffman, Michaela et al. (2018) Gray and white matter integrity influence TMS signal propagation: a multimodal evaluation in cocaine-dependent individuals. Sci Rep 8:3253
Giannotti, Giuseppe; Barry, Sarah M; Siemsen, Ben M et al. (2018) Divergent Prelimbic Cortical Pathways Interact with BDNF to Regulate Cocaine-seeking. J Neurosci 38:8956-8966
Siemsen, Ben M; Lombroso, Paul J; McGinty, Jacqueline F (2018) Intra-prelimbic cortical inhibition of striatal-enriched tyrosine phosphatase suppresses cocaine seeking in rats. Addict Biol 23:219-229
Brown, Robyn Mary; Kupchik, Yonatan Michael; Spencer, Sade et al. (2017) Addiction-like Synaptic Impairments in Diet-Induced Obesity. Biol Psychiatry 81:797-806
Bobadilla, Ana-Clara; Garcia-Keller, Constanza; Heinsbroek, Jasper A et al. (2017) Accumbens Mechanisms for Cued Sucrose Seeking. Neuropsychopharmacology 42:2377-2386
Spencer, Sade; Garcia-Keller, Constanza; Roberts-Wolfe, Douglas et al. (2017) Cocaine Use Reverses Striatal Plasticity Produced During Cocaine Seeking. Biol Psychiatry 81:616-624

Showing the most recent 10 out of 180 publications