Because life expectancy is increasing in the United States, The number of persons aged sixty-five or older will be more than that of persons aged nineteen years or younger by mid-1990's. It is well recognized that. as persons grow older, the incidence of root surface caries increases. Less well recognized however. is the observation that the output of the submandibular and sublingual glands declines with age. This phenomenon may be further complicated by the extensive use of prescription drugs that have a hyposalivatory effect. It is estimated that 75% of the population aged 65 or older take one or more prescription drugs with a hyposalivatory effect. The effect that age and/or drugs have on composition of saliva and in particular the submandibular and sublingual saliva remains largely unexplored. The influence of chronic ingestion of hyposalivator drugs on coronal and root surface caries development has not been examined eve though it is recognized that a major reduction in the total volume of saliva lead to enhanced levels of caries. In the proposed studies. we will examine the influence of administration of the commonly prescribed atropine (cholinergic antagonist). propranolol (beta adrenergic antagonist), and amitriptyline (cholinergic and alpha adrenergic inhibitor) administered by means of osmotic pumps on caries development, salivary output, and composition of saliva in rats of various ages. We will also explore the effects of these regimens on the microbial flora of the mouth. In addition, the ability of saliva collected from ducts and particular salivary constituents to bind to HA and the ability of microorganisms to bind to HA will be explored. The results of these studies will help to clarify the influence of drugs on salivary gland physiology and biochemistry and caries development. They will also aid in identifying particular constituents in salivassociated with caries resistance and help to classify groups of drugs which may enhance particularly caries promoting because of their effect on the composition of saliva. Equally, the study could lead to the development of a drug-that may enhance the level of caries protection exerted by saliva.

National Institute of Health (NIH)
National Institute of Dental & Craniofacial Research (NIDCR)
Specialized Center (P50)
Project #
Application #
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Rochester
United States
Zip Code
Miguel, J C; Bowen, W H; Pearson, S K (1997) Effects of frequency of exposure to iron-sucrose on the incidence of dental caries in desalivated rats. Caries Res 31:238-43
Vacca-Smith, A M; Venkitaraman, A R; Schilling, K M et al. (1996) Characterization of glucosyltransferase of human saliva adsorbed onto hydroxyapatite surfaces. Caries Res 30:354-60
Venkitaraman, A R; Vacca-Smith, A M; Kopec, L K et al. (1995) Characterization of glucosyltransferaseB, GtfC, and GtfD in solution and on the surface of hydroxyapatite. J Dent Res 74:1695-701
Van Wuyckhuyse, B C; Perinpanayagam, H E; Bevacqua, D et al. (1995) Association of free arginine and lysine concentrations in human parotid saliva with caries experience. J Dent Res 74:686-90
Perinpanayagam, H E; Van Wuyckhuyse, B C; Ji, Z S et al. (1995) Characterization of low-molecular-weight peptides in human parotid saliva. J Dent Res 74:345-50
Adair, S M; Leverett, D H; Shaffer, C L (1994) Interexaminer agreement for readings of dip slide tests for salivary mutans streptococci and lactobacilli. Caries Res 28:123-6
Zhang, G H; Melvin, J E (1994) Intracellular Mg2+ movement during muscarinic stimulation. Mg2+ uptake by the intracellular Ca2+ store in rat sublingual mucous acini. J Biol Chem 269:10352-6
O'Connell, A C; Bowen, W H (1994) Composition and flow rate of saliva and caries development in young rats following administration of lithium. Caries Res 28:342-7
O'Connell, A C; Pearson, S K; Bowen, W H (1994) Pilocarpine alters caries development in partially-desalivated rats. J Dent Res 73:637-43
Watson, G E; Culp, D J (1994) Muscarinic cholinergic receptor subtypes in rat sublingual glands. Am J Physiol 266:C335-42

Showing the most recent 10 out of 69 publications