Core - Cytokine Analysis

Beagley, Kenneth W.

Abstract

The Cytokine Core will assist RCOB investigators with cytokine analysis. Analyses will include cytokine determination of media and fluids by bioassays and ELISA, and cytokine mRNA expression by Dot/Northern analysis, S1 analysis, RNAse protection analysis, RT-PCR and in situ hybridization. the core will also provide advice and expertise in the planning of cytokine-related experiments. the core at this time has available cDNA probes for human (IL-1 through IL-10, IFN-gamma, TGF- alpha, TNF-alpha/beta) and for murine (IL-1 through IL-10, TNF- alpha/beta, TGF-alpha, IFN-gamma) cytokines. Bioassays presently available include IL-1 (human and mouse). In addition, ELISA's for murine IL-5 and IFN-gamma are also available. Neutralizing antibodies used in these bioassays will also be available to researchers at UAB through the Core Facility. The core is fully equipped with instrumentation for these analyses and has developed the necessary expertise to conduct the proposed studies. The Core will be used extensively by Projects 8 and 5 and occasionally by Projects 4 and 3.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Dental & Craniofacial Research (NIDCR)
• Type: Specialized Center (P50)
• Project #: 5P50DE008228-09
• Application #: 5210118
• Support Year: 9
• Fiscal Year: 1996

Publications

Windsor, L Jack; Steele, Darin L (2010) Expression of recombinant matrix metalloproteinases in Escherichia coli. Methods Mol Biol 622:67-81

Katz, Jannet; Yang, Qiu-Bo; Zhang, Ping et al. (2002) Hydrolysis of epithelial junctional proteins by Porphyromonas gingivalis gingipains. Infect Immun 70:2512-8

Smith, P D; Smythies, L E; Mosteller-Barnum, M et al. (2001) Intestinal macrophages lack CD14 and CD89 and consequently are down-regulated for LPS- and IgA-mediated activities. J Immunol 167:2651-6

Aznavoorian, S; Moore, B A; Alexander-Lister, L D et al. (2001) Membrane type I-matrix metalloproteinase-mediated degradation of type I collagen by oral squamous cell carcinoma cells. Cancer Res 61:6264-75

Hirschfeld, M; Weis, J J; Toshchakov, V et al. (2001) Signaling by toll-like receptor 2 and 4 agonists results in differential gene expression in murine macrophages. Infect Immun 69:1477-82

Windsor, L J; Steele, D L (2001) Expression of recombinant matrix metalloproteinases in Escherichia coli. Methods Mol Biol 151:191-205

Caterina, J J; Shi, J; Kozak, C A et al. (2000) Characterization, expression analysis and chromosomal mapping of mouse matrix metalloproteinase-19 (MMP-19). Mol Biol Rep 27:73-9

Foster, K W; Frost, A R; McKie-Bell, P et al. (2000) Increase of GKLF messenger RNA and protein expression during progression of breast cancer. Cancer Res 60:6488-95

Caterina, J; Shi, J; Sun, X et al. (2000) Cloning, characterization, and expression analysis of mouse enamelysin. J Dent Res 79:1697-703

Caterina, J J; Yamada, S; Caterina, N C et al. (2000) Inactivating mutation of the mouse tissue inhibitor of metalloproteinases-2(Timp-2) gene alters proMMP-2 activation. J Biol Chem 275:26416-22