Abstract

This project seeks to understand the molecular basis of the interactions of P. gingivalis, a major periodontal pathogen, with the extracellular matrix component, fibronectin. This interaction takes place in an iron- poor environment, but is destroyed in an iron-rich environment, in which protease activity is elevated. Bifunctional crosslinking agents will be used to determine the surface receptor on P. gingivalis. In a second set of experiments, recombinant domains of fibronectin produced in collaboration with Dr. Paul Johnson (Project 2 and Core 9001) will be used in competition experiments to establish which domain(s) of fibronectin interact with the surface of P. gingivalis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Specialized Center (P50)
Project #
5P50DE010306-04
Application #
5210202
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Kamiya, Nobuhiro; Jikko, Akitoshi; Kimata, Koji et al. (2002) Establishment of a novel chondrocytic cell line N1511 derived from p53-null mice. J Bone Miner Res 17:1832-42
Choy, L; Skillington, J; Derynck, R (2000) Roles of autocrine TGF-beta receptor and Smad signaling in adipocyte differentiation. J Cell Biol 149:667-82
Suen, P W; Ilic, D; Caveggion, E et al. (1999) Impaired integrin-mediated signal transduction, altered cytoskeletal structure and reduced motility in Hck/Fgr deficient macrophages. J Cell Sci 112 ( Pt 22):4067-78
Johnson, P W; Lancero, H (1999) Function of gingival fibroblasts and periodontal ligament cells in the presence of methyl mercaptan. Quintessence Int 30:343-9
Gerber, H P; Vu, T H; Ryan, A M et al. (1999) VEGF couples hypertrophic cartilage remodeling, ossification and angiogenesis during endochondral bone formation. Nat Med 5:623-8
Bullard, K M; Lund, L; Mudgett, J S et al. (1999) Impaired wound contraction in stromelysin-1-deficient mice. Ann Surg 230:260-5
Filvaroff, E; Erlebacher, A; Ye, J et al. (1999) Inhibition of TGF-beta receptor signaling in osteoblasts leads to decreased bone remodeling and increased trabecular bone mass. Development 126:4267-79
Miettinen, P J; Chin, J R; Shum, L et al. (1999) Epidermal growth factor receptor function is necessary for normal craniofacial development and palate closure. Nat Genet 22:69-73
Jikko, A; Harris, S E; Chen, D et al. (1999) Collagen integrin receptors regulate early osteoblast differentiation induced by BMP-2. J Bone Miner Res 14:1075-83
Iwasaki, M; Jikko, A; Le, A X (1999) Age-dependent effects of hedgehog protein on chondrocytes. J Bone Joint Surg Br 81:1076-82

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