Abstract

The objectives of this work are to obtain molecular information on the structure and function of a chloride channel, and to extend the work to the isolation of the gene for the transporter. The methods to be used are membrane isolation, reconstitution, transport specific fractionation, protein chemistry, and recombinant DNA techniques.
The aim i s to develop methods to study a chloride channel from animal tissues (bovine trachea and electric ray electroplax), and to use the cDNA for this protein to obtain information about the human protein(s).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Specialized Center (P50)
Project #
5P50DK039249-04
Application #
3876158
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
Brigham and Women's Hospital
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Greenfeld, Z; Stillman, I E; Brezis, M et al. (1997) Medullary injury in the ageing rat kidney: functional-morphometric correlations. Eur J Clin Invest 27:346-51
Stillman, I E; Brezis, M; Heyman, S N et al. (1994) Effects of salt depletion on the kidney: changes in medullary oxygenation and thick ascending limb size. J Am Soc Nephrol 4:1538-45
Brugnara, C; De Franceschi, L; Alper, S L (1993) Ca(2+)-activated K+ transport in erythrocytes. Comparison of binding and transport inhibition by scorpion toxins. J Biol Chem 268:8760-8
Heyman, S N; Stillman, I E; Brezis, M et al. (1993) Chronic amphotericin nephropathy: morphometric, electron microscopic, and functional studies. J Am Soc Nephrol 4:69-80
Hallaq, H; Smith, T W; Leaf, A (1992) Modulation of dihydropyridine-sensitive calcium channels in heart cells by fish oil fatty acids. Proc Natl Acad Sci U S A 89:1760-4
Heyman, S N; Clark, B A; Kaiser, N et al. (1992) In-vivo and in-vitro studies on the effect of amphotericin B on endothelin release. J Antimicrob Chemother 29:69-77
Heyman, S N; Clark, B A; Kaiser, N et al. (1992) Radiocontrast agents induce endothelin release in vivo and in vitro. J Am Soc Nephrol 3:58-65
Chatzipanteli, K; Rudolph, S; Axelrod, L (1992) Coordinate control of lipolysis by prostaglandin E2 and prostacyclin in rat adipose tissue. Diabetes 41:927-35
Diaz-Gallo, C; Moscovitch-Lopatin, M; Strom, T B et al. (1992) An anergic, islet-infiltrating T-cell clone that suppresses murine diabetes secretes a factor that blocks interleukin 2/interleukin 4-dependent proliferation. Proc Natl Acad Sci U S A 89:8656-60
Heyman, S N; Brezis, M; Epstein, F H et al. (1992) Effect of glycine and hypertrophy on renal outer medullary hypoxic injury in ischemia reflow and contrast nephropathy. Am J Kidney Dis 19:578-86

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