Although there have been a number of studies on experimental bladder outlet obstruction, the majority of them have been of limited scope. This section of the proposed study will systematically evaluate the in vitro functional and biochemical changes that result from bladder outlet obstruction of varying magnitude and duration, and specifically evaluate the following parameters in obstructed vs normal cats: (1) in vitro cystometry and the functional ability of the bladder to empty; (2) secondary changes in ureteral function and biochemistry; (3) contractility of isolated strips of bladder dome, body, base and urethra; (4) correlation of the contractile response of the strips to specific adrenergic and cholinergic agonists with specific autonomic receptors densities as determined by radioligand receptor analysis; and (5) intracellular concentrations of ATP, CP, DNA, RNA, lipids, glycogen, elastin and collagen. The results will be correlated with data obtained from in vivo studies (Section I) and morphologic/morphometric evaluation of the bladder and ureter (Section II), in order to determine the pathogenetic mechanism(s) responsible for vesical and ureteral dysfunction in obstructive uropathy.

Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Upstate Medical University
United States
Zip Code
Levin, R M; Haugaard, N; Eika, B et al. (1994) Comparative biochemical characteristics of the cat and rabbit urinary bladder. Neurourol Urodyn 13:307-14
Yoon, J Y; Zderic, S A; Duckett, J W et al. (1994) Effect of partial outlet obstruction on the biphasic response to field stimulation at different concentrations of calcium. Pharmacology 49:167-72
Levin, R M; Monson, F C; Longhurst, P A et al. (1994) Rabbit as a model of urinary bladder function. Neurourol Urodyn 13:119-35
Zhao, Y; Wein, A J; Levin, R M (1993) Role of calcium in mediating the biphasic contraction of the rabbit urinary bladder. Gen Pharmacol 24:727-31
Hess, M E; Barasha, B; Winters, S et al. (1993) Effect of thyroxine on urinary bladder autonomic receptor densities and contractility. Pharmacology 46:248-53
Samadzadeh, S; Tong, Y C; Wein, A J et al. (1992) Influence of the bathing medium on the contractile responses of the rabbit urinary bladder. Pharmacology 45:99-106
Longhurst, P A; Eika, B; Leggett, R E et al. (1992) Urinary bladder function in the tight-skin mouse. J Urol 148:1611-4
Bilgen, A; Wein, A J; Zhao, Y et al. (1992) Effects of anoxia on the biphasic response of isolated strips of rabbit bladder to field stimulation, bethanechol, methoxamine and KCl. Pharmacology 44:283-9
Levin, R M; Haugaard, N; Packard, D et al. (1992) Effect of ryanodine on mitochondrial respiration. Pharmacology 45:117-20
Tammela, T L; Wein, A J; Levin, R M (1992) Effect of tetrodotoxin on the phasic and tonic responses of isolated rabbit urinary bladder smooth muscle to field stimulation. J Urol 148:1937-40

Showing the most recent 10 out of 27 publications