Core - Homologous Recombination Facility

Thomas, Kirk R.

Abstract

The Homologous Recombination Core will make available the technology of targeted gene disruption of homologous recombination. 1. Maintain a full complement of genomic libraries, selectable markers, and bacterial and phage vectors required for gene modification in mouse embryonic stem (ES) cells and other rodent and human cultured cell lines. 2. Provide consultation services regarding all steps in the gene modification protocols including vector design, selection strategies and recombinant identification. 3. Provide an ES cell manipulation service. 4. Develop new technology in four areas: (a) isolation of new ES cell lines (b) adaptation of novel cell/embryo methods (c) development of gene targeting in non-ES lines (d) development of a conditional-mutant system applicable to the adult mouse

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
• Type: Specialized Center (P50)
• Project #: 5P50DK049219-03
• Application #: 5210911
• Support Year: 3
• Fiscal Year: 1996

Publications

Prasher, J M; Elenitoba-Johnson, K S; Kelley, L L (2001) Loss of p53 tumor suppressor function is required for in vivo progression of Friend erythroleukemia. Oncogene 20:2946-55

Mojica, M P; Perry, S S; Searles, A E et al. (2001) Phenotypic distinction and functional characterization of pro-B cells in adult mouse bone marrow. J Immunol 166:3042-51

Lok, C N; Ponka, P (2000) Identification of an erythroid active element in the transferrin receptor gene. J Biol Chem 275:24185-90

Hsieh, F F; Barnett, L A; Green, W F et al. (2000) Cell cycle exit during terminal erythroid differentiation is associated with accumulation of p27(Kip1) and inactivation of cdk2 kinase. Blood 96:2746-54

Wiesmann, A; Kim, M; Georgelas, A et al. (2000) Modulation of hematopoietic stem/progenitor cell engraftment by transforming growth factor beta. Exp Hematol 28:128-39

Spangrude, G J; Cooper, D D (2000) Paradigm shifts in stem-cell biology. Semin Hematol 37:10-Mar

Wiesmann, A; Phillips, R L; Mojica, M et al. (2000) Expression of CD27 on murine hematopoietic stem and progenitor cells. Immunity 12:193-9

Searles, A E; Pohlmann, S J; Pierce, L J et al. (2000) Rapid, B lymphoid-restricted engraftment mediated by a primitive bone marrow subpopulation. J Immunol 165:67-74

Wiesmann, A; Spangrude, G J (1999) Marrow engraftment of hematopoietic stem and progenitor cells is independent of Galphai-coupled chemokine receptors. Exp Hematol 27:946-55

Perry, S S; Kim, M; Spangrude, G J (1999) Direct effects of cyclosporin A on proliferation of hematopoietic stem and progenitor cells. Cell Transplant 8:339-44