The Proteomics Core (Core B) offers a full array of proteomics and biological mass spectrometry capabilities to members ofthe CCHMC Pediatric Center of Excellence in Nephrology. The capabilities offered range from simple quality assurance analysis of isolated proteins and pepfides, to more complex comparative profiling of proteins from two or more biological conditions, and finally to the development of new technologies like global phosphoprotein profiling and mass spectrometry-based technologies for high throughput inhibitor screening. We will focus on the following 4 specific aims: (1) Quantitafion and identification of protein changes by 2D gel-based methods;(2) Quantitation and identification of protein changes by isotope tagging and nanoLC-MS/MS;(3) Identification of protein biomarkers detected from biofluids;(4) Provision of dedicated and expert Biostatisfical and Bioinformafic support for data analysis. In addifion to these specific funcfions, the full capabilities and expertise ofthe Proteomics Core will be available to Center members as needed. These include advanced measures of protein and peptide quality assurances, identification of protein interacting complexes, and mapping of post-translational modifications. Another goal is to provide training opportunifies in proteomics and biological mass spectrometry. Anticipated users of Core B include Goldstein (Research Project 1), Potter (Research Project 2), Brunner (Research Project 3), Basu (Pilot and Feasibility Project 1) and Jodele (Pilot and Feasibility Project 2). Core B is intimately related to the overall goals of this center grant, and is aimed at performing crifical translafional studies in pediatric nephrologic diseases that suffer from a major unmet need. It will direcfiy address the identified research objecfives in the RFA-DK-11-009 to study pediatric glomerular diseases with a poor prognosis, namely FSGS (Potter) and lupus nephritis (Brunner), and to study acute kidney injury (Goldstein, Basu, Jodele). Our long term goal is to develop and extend expertise in all aspects of renal proteomics, in order to become a local, regional, and national resource for the Nephrology community as a whole.

Public Health Relevance

Pediatric kidney diseases due to acute kidney injury, focal segmental glomerulosclerosis, and lupus nephrifis contribute to an enormous major impact on the U.S. public health and a major financial burden. The Protomics Core ofthis Center of Excellence in Nephrology will provide critical proteomic and informafic tools for multiple investigators to advance studies on these three disease states to change their dismal outcome

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Specialized Center (P50)
Project #
5P50DK096418-03
Application #
8731220
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
3
Fiscal Year
2014
Total Cost
Indirect Cost
City
Cincinnati
State
OH
Country
United States
Zip Code
45229
Bennett, Michael R; Pyles, Olivia; Ma, Qing et al. (2018) Preoperative levels of urinary uromodulin predict acute kidney injury after pediatric cardiopulmonary bypass surgery. Pediatr Nephrol 33:521-526
Albert, Christian; Albert, Annemarie; Bellomo, Rinaldo et al. (2018) Urinary neutrophil gelatinase-associated lipocalin-guided risk assessment for major adverse kidney events after open-heart surgery. Biomark Med 12:975-985
Basu, Rajit K; Kaddourah, Ahmad; Goldstein, Stuart L et al. (2018) Assessment of a renal angina index for prediction of severe acute kidney injury in critically ill children: a multicentre, multinational, prospective observational study. Lancet Child Adolesc Health 2:112-120
Jotwani, Vasantha; Scherzer, Rebecca; Glidden, David V et al. (2018) Pre-exposure Prophylaxis With Tenofovir Disoproxil Fumarate/Emtricitabine and Kidney Tubular Dysfunction in HIV-Uninfected Individuals. J Acquir Immune Defic Syndr 78:169-174
Valiente-Alandi, IƱigo; Potter, Sarah J; Salvador, Ane M et al. (2018) Inhibiting Fibronectin Attenuates Fibrosis and Improves Cardiac Function in a Model of Heart Failure. Circulation 138:1236-1252
Jotwani, Vasantha; Katz, Ronit; Ix, Joachim H et al. (2018) Urinary Biomarkers of Kidney Tubular Damage and Risk of Cardiovascular Disease and Mortality in Elders. Am J Kidney Dis 72:205-213
Drake, Keri A; Adam, Mike; Mahoney, Robert et al. (2018) Disruption of Hox9,10,11 function results in cellular level lineage infidelity in the kidney. Sci Rep 8:6306
Forster, Catherine S; Jackson, Elizabeth; Ma, Qing et al. (2018) Predictive ability of NGAL in identifying urinary tract infection in children with neurogenic bladders. Pediatr Nephrol 33:1365-1374
Magella, Bliss; Mahoney, Robert; Adam, Mike et al. (2018) Reduced Abd-B Hox function during kidney development results in lineage infidelity. Dev Biol 438:84-93
Potter, S Steven (2018) Single-cell RNA sequencing for the study of development, physiology and disease. Nat Rev Nephrol 14:479-492

Showing the most recent 10 out of 124 publications