The Cincinnati Children's Hospital Medical Center Biomarker Core (Core C) with provide support for all aspects of biomarker research to the Pediatric Centers of Excellence in Nephrology. These services range from specimen handling, biofluid profiling and discovery, to assay development and biomarker validation, to commercialization. The overarching goal of Biomarker Core C is to provide an innovative service for the seamless discovery, translation, validation, and commercialization of novel biomarkers and therapeutic targets for human diseases, in collaboration with other cores. The Biomarker Core, founded by Prasad Devarajan, MD, and co-directed by Michael Bennett, PhD, is housed in the Division of Nephrology and Hypertension at Cincinnati Children's Research Foundation. Through collaborative and fee-for-service arrangements, the lab has grown into a wide-ranging service and research provider for clinicians and investigators across the globe. Over the past three years, we have performed well over 200,000 assays for AKI and CKD biomarkers for investigators worldwide. Dr. Bennett has a broad background in cell biology, molecular biology and biochemisty. He was recruited to join Dr. Devarajan's team 5 years ago to direct the Biomarker Core and supervise the research operations of the Nephrology Clinical Laboratory. The seamless connection to the CAP and CLIA certified clinical laboratory provides the Biomarker Core access to a diverse menu of advanced clinical assays on high throughput standardized clinical laboratory platforms. Several of the research projects in this application (Goldstein, Brunner, Basu, Jodele,) require the services of the Biomarker Core to fulfill their Aims. To serve the needs of this team of investigators, the Biomarker Core will focus on these two specific aims: (1) To provide high throughput Clinical Biofluid Profiling Services using SELDI-TOF (list investigators who will use this), and (2) To provide services pertaining to Biomarker measurement and validation (including Design of ELISA and Western Blots, Measurement of markers of AKI and CKD, as well as consultation for biomarker statistics and clinical trials design).

Public Health Relevance

Pediatric kidney diseases due to acute kidney injury, focal segmental glomerulosclerosis, and lupus nephritis contribute to an enormous major impact on the U.S. public health and a major financial burden. The Biomarker Core of this Center of Excellence will provide critical biomarker discovery and validation expertise for multiple investigators to advance studies on these three disease states to change their dismal outcome

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Specialized Center (P50)
Project #
5P50DK096418-03
Application #
8731221
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
3
Fiscal Year
2014
Total Cost
Indirect Cost
City
Cincinnati
State
OH
Country
United States
Zip Code
45229
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Jotwani, Vasantha; Scherzer, Rebecca; Glidden, David V et al. (2018) Pre-exposure Prophylaxis With Tenofovir Disoproxil Fumarate/Emtricitabine and Kidney Tubular Dysfunction in HIV-Uninfected Individuals. J Acquir Immune Defic Syndr 78:169-174
Valiente-Alandi, Iñigo; Potter, Sarah J; Salvador, Ane M et al. (2018) Inhibiting Fibronectin Attenuates Fibrosis and Improves Cardiac Function in a Model of Heart Failure. Circulation 138:1236-1252
Jotwani, Vasantha; Katz, Ronit; Ix, Joachim H et al. (2018) Urinary Biomarkers of Kidney Tubular Damage and Risk of Cardiovascular Disease and Mortality in Elders. Am J Kidney Dis 72:205-213
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Forster, Catherine S; Jackson, Elizabeth; Ma, Qing et al. (2018) Predictive ability of NGAL in identifying urinary tract infection in children with neurogenic bladders. Pediatr Nephrol 33:1365-1374
Magella, Bliss; Mahoney, Robert; Adam, Mike et al. (2018) Reduced Abd-B Hox function during kidney development results in lineage infidelity. Dev Biol 438:84-93
Potter, S Steven (2018) Single-cell RNA sequencing for the study of development, physiology and disease. Nat Rev Nephrol 14:479-492
Greenberg, Jason H; Devarajan, Prasad; Thiessen-Philbrook, Heather R et al. (2018) Kidney injury biomarkers 5 years after AKI due to pediatric cardiac surgery. Pediatr Nephrol 33:1069-1077
Benoit, Stefanie Woolridge; Devarajan, Prasad (2018) Acute kidney injury: emerging pharmacotherapies in current clinical trials. Pediatr Nephrol 33:779-787

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