Air pollution has long been associated with an increased risk of cardiovascular disease, however the exactmechanisms that underlie the pathogenesis of air pollution induced myocardial infarction remainincompletely understood. Although numerous studies have examined vascular effects as the primary causeof myocardial injury, only a few studies have investigated for direct toxic effects of inhaled pollutants onmyocardial cells. Some of these studies have suggested an increased incidence of myocardial apoptosis andfibrosis after experimental exposure, raising the possibility that some of the arrhythmogenic and myopathiceffects seen in clinical populations may ensue from direct myocardial injury. Accordingly, we propose thefollowing specific aims:
Aim 1 : Determine whether exposure to diesel exhaust (DE) predisposes to hypertrophy, heart failure,apoptosis, fibrosis and myocardial infarction in vivo and in vitro in experimental mouse models.We will expose wild type mice to diesel exhaust and then assess for LV function and cardiac hypertrophy.We will also assess for apoptosis and fibrosis and will challenge exposed mice with hypertrophic stimuli todetermine whether the mice are predisposed to hypertrophy and heart failure after exposure.
Aim 2 : Determine whether CHF1/Hey2, an important regulator of myocardial hypertrophy and heart failure,affects the pathological response to diesel exhaust.We propose to examine whether myocardial CHF1/Hey2 expression is altered in experimental animalsexposed to diesel exhaust, and then will evaluate whether loss of function or gain of function in mice canalter their experimental response to diesel exhaust.
Aim 3 : Determine whether serum-borne mediators in exposed animals and patients induce myocardialtoxicity and identify potential diesel exhaust-induced transcriptional mechanisms.We will treat cultured myocytes from unexposed animals with serum from exposed animals and patients andmeasure cellular hypertrophy and apoptosis, to determine whether there are soluble mediators in exposedpatient serum that predispose to either of these pathological processes. The transcriptional basis foralteration in hypertrophic or apoptotic responses would be further examined by genomic analysis.
Showing the most recent 10 out of 85 publications
