The goal of this research program is to understand wound healing and its failure. In preliminary experiments, we analyzed wound fluid from burn wounds, chronic skin ulcers, and mastectomy wounds. The results showed that wound fluid can be used as a non-invasive method to learn about wound repair. During the next four years, we will build on the preliminary studies to answer important questions about the proteinases, adhesion proteins, and growth factors found in would fluid and their biological functions.
Specific aims of the project are as follows: to learn if elastase is responsible for degradation of fibronectin in burn wound fluid and chronic ulcer fluid and if there is a defect in the proteinase inhibitor system; to learn if fibronectin fragments in wound fluid stimulate proteinase secretion or have endogenous proteinase activity; to learn if """"""""activated"""""""" metalloproteinases in chronic ulcer and burn wound fluid are biologically active; to learn if treatment of mastectomy fluid with """"""""inflammatory"""""""" enzymes results in metalloproteinase activation and fibronectin degradation similar to that seen in chronic wound fluid or burn wound fluid; to identify the burn wound fluid growth factor and other factors that modify keratinocyte migration or fibroblast contraction; and to further correlate the relationship between wound fluid composition and clinical healing.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Specialized Center (P50)
Project #
Application #
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
United States
Zip Code
Lin, Keng-Mean; Hu, Wei; Troutman, Ty Dale et al. (2014) IRAK-1 bypasses priming and directly links TLRs to rapid NLRP3 inflammasome activation. Proc Natl Acad Sci U S A 111:775-80
Lu, Dongmei; Sun, Hui-qiao; Wang, Hanzhi et al. (2012) Phosphatidylinositol 4-kinase II? is palmitoylated by Golgi-localized palmitoyltransferases in cholesterol-dependent manner. J Biol Chem 287:21856-65
Sun, Yi; Dandekar, Radhika D; Mao, Yuntao S et al. (2011) Phosphatidylinositol (4,5) bisphosphate controls T cell activation by regulating T cell rigidity and organization. PLoS One 6:e27227
Hassan, Monique; Pham, Tam N; Cuschieri, Joseph et al. (2011) The association between the transfusion of older blood and outcomes after trauma. Shock 35:3-8
Gatson, J W; Simpkins, J W; Yi, K D et al. (2011) Aromatase is increased in astrocytes in the presence of elevated pressure. Endocrinology 152:207-13
Huebinger, Ryan M; Gomez, Ruben; McGee, Daphne et al. (2010) Association of mitochondrial allele 4216C with increased risk for sepsis-related organ dysfunction and shock after burn injury. Shock 33:19-23
Wang, Lin; Quan, Jiexia; Johnston, William E et al. (2010) Age-dependent differences of interleukin-6 activity in cardiac function after burn complicated by sepsis. Burns 36:232-8
Huebinger, Ryan M; Rivera-Chavez, Fernando; Chang, Ling-Yu et al. (2010) IL-10 polymorphism associated with decreased risk for mortality after burn injury. J Surg Res 164:e141-5
Huebinger, Ryan M; Garner, Harold R; Barber, Robert C (2010) Pathway genetic load allows simultaneous evaluation of multiple genetic associations. Burns 36:787-92
Chen, Mark Z; Zhu, Xiaohui; Sun, Hui-Qiao et al. (2009) Oxidative stress decreases phosphatidylinositol 4,5-bisphosphate levels by deactivating phosphatidylinositol- 4-phosphate 5-kinase beta in a Syk-dependent manner. J Biol Chem 284:23743-53

Showing the most recent 10 out of 70 publications