The PET and Mass Spectrometry (MS) Core provides the Center with the capability to study critical phenomena at the tissue, cellular, and genetic levels in both humans and living animals;non-invasively with PET. The core allows complex studies to be performed by the Human Subjects and Animal Research Cores at the MGH and SHC and provides investigators with the capability to apply PET and MS technology to test scientific hypotheses without the need to become experts in the technologies themselves. Conventional PET cameras and pPET devices provide the ability to study animals more than once, allows each animal to serve as its own control, and allows interventional strategies to be followed over time. Furthermore, identical studies can also be conducted non-invasively directly in normal human volunteers and patients. The DPET is mobile and makes imaging skeletal muscle possible in acutely ill intensive care patients. Further development of molecular imaging methodologies will enable imaging of fatty acid metabolism, mitochondrial function, gene expression and apoptosis;initially in mice and ultimately in humans. Services offered by the PET component ofthe core, include: (1) Design and development of new imaging agents. (2) Routine production of existing PET agents. (3) Routine execution of existing imaging protocols and data analyses. (4) Development of new instrumentation. The MS component of the core supports research that allows our investigators to apply stable isotope tracers for studying: amino acid, glucose and lipid metabolism, nitrate/nitric oxide, protein turnover, proteomics and substrate kinetics/oxidation. Services to the center, include: (1) Timely and accurate determinations of isotope enrichments of tracers and their derivatives in biological samples. (2) Development of new methods for isotope analyses of compounds in blood, tissues, and urine to meet the needs of our investigators. (3) Quality control for all analysis conducted in support of studies that use stable isotopes. This facility enables research in all 4 projects and funds to support these activities directly are included in each project.
Ths core allows complex studies to be performed by the Human Subjects and Animal Research Cores at the MGH and SHC and provides investigators with the capability to apply PET and MS technology to test scientific hypotheses without the need to become experts in the technologies themselves.
|Tao, Rongya; Wang, Caixia; Stöhr, Oliver et al. (2018) Inactivating hepatic follistatin alleviates hyperglycemia. Nat Med 24:1058-1069|
|Nakazawa, Harumasa; Chang, Kyungho; Shinozaki, Shohei et al. (2017) iNOS as a Driver of Inflammation and Apoptosis in Mouse Skeletal Muscle after Burn Injury: Possible Involvement of Sirt1 S-Nitrosylation-Mediated Acetylation of p65 NF-?B and p53. PLoS One 12:e0170391|
|Frydman, Galit H; Marini, Robert P; Bakthavatchalu, Vasudevan et al. (2017) Local and Systemic Changes Associated with Long-term, Percutaneous, Static Implantation of Titanium Alloys in Rhesus Macaques (Macaca mulatta). Comp Med 67:165-175|
|Khan, Mohammed A S; Khan, Mohammed F; Kashiwagi, Shizuka et al. (2017) An ALPHA7 Nicotinic Acetylcholine Receptor Agonist (GTS-21) Promotes C2C12 Myonuclear Accretion in Association with Release of Interleukin-6 (IL-6) and Improves Survival in Burned Mice. Shock 48:227-235|
|Li, Peng; Tompkins, Ronald G; Xiao, Wenzhong et al. (2017) KERIS: kaleidoscope of gene responses to inflammation between species. Nucleic Acids Res 45:D908-D914|
|Kashiwagi, Shizuka; Khan, Mohammed A S; Yasuhara, Shingo et al. (2017) Prevention of Burn-Induced Inflammatory Responses and Muscle Wasting by GTS-21, a Specific Agonist for ?7 Nicotinic Acetylcholine Receptors. Shock 47:61-69|
|Ueki, Ryusuke; Liu, Li; Kashiwagi, Shizuka et al. (2016) Role of Elevated Fibrinogen in Burn-Induced Mitochondrial Dysfunction: Protective Effects of Glycyrrhizin. Shock 46:382-9|
|Agarwal, Shailesh; Loder, Shawn; Brownley, Cameron et al. (2016) Inhibition of Hif1? prevents both trauma-induced and genetic heterotopic ossification. Proc Natl Acad Sci U S A 113:E338-47|
|Shank, Erik S; Martyn, Jeevendra A; Donelan, Mathias B et al. (2016) Ultrasound-Guided Regional Anesthesia for Pediatric Burn Reconstructive Surgery: A Prospective Study. J Burn Care Res 37:e213-7|
|Copps, Kyle D; Hançer, Nancy J; Qiu, Wei et al. (2016) Serine 302 Phosphorylation of Mouse Insulin Receptor Substrate 1 (IRS1) Is Dispensable for Normal Insulin Signaling and Feedback Regulation by Hepatic S6 Kinase. J Biol Chem 291:8602-17|
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