Abstract

The objective of this project is to develop one or more microbial overexpression systems for Mycoplasma genitalium/pneumoniae protein genes or their homologs in other organisms, and to purify those proteins for structural studies. Four groups of gene for cloning are: 1. The soluble proteins of the Mycoplasmas or their homologs from other organisms for which cellular functions have been inferred based on sequence similarities but no molecular functions and/or structural information are available; 2. The """"""""hypothetical"""""""" Mycoplasma genes for which there are neither inferred functions nor structural homologs; 3. The Mycoplasma proteins or their homologs that belong to sequence families with multiple members and/or important cellular function; 4. The soluble domains of simple membrane proteins of the Mycoplasmas of their homologs.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
5P50GM062412-03
Application #
6654604
Study Section
Special Emphasis Panel (ZGM1)
Project Start
2002-09-01
Project End
2003-08-31
Budget Start
Budget End
Support Year
3
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
University of California Berkeley
Department
Type
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704

  Publications

Jun, Se-Ran; Sims, Gregory E; Wu, Guohong A et al. (2010) Whole-proteome phylogeny of prokaryotes by feature frequency profiles: An alignment-free method with optimal feature resolution. Proc Natl Acad Sci U S A 107:133-8
Lee, Jonas; Kim, Sung-Hou (2009) High-throughput T7 LIC vector for introducing C-terminal poly-histidine tags with variable lengths without extra sequences. Protein Expr Purif 63:58-61
Wu, Guohong Albert; Jun, Se-Ran; Sims, Gregory E et al. (2009) Whole-proteome phylogeny of large dsDNA virus families by an alignment-free method. Proc Natl Acad Sci U S A 106:12826-31
Pereira, Jose Henrique; Kim, Sung-Hou (2009) Structure of human Brn-5 transcription factor in complex with CRH gene promoter. J Struct Biol 167:159-65
Sims, Gregory E; Jun, Se-Ran; Wu, Guohong Albert et al. (2009) Whole-genome phylogeny of mammals: evolutionary information in genic and nongenic regions. Proc Natl Acad Sci U S A 106:17077-82
Sims, Gregory E; Jun, Se-Ran; Wu, Guohong A et al. (2009) Alignment-free genome comparison with feature frequency profiles (FFP) and optimal resolutions. Proc Natl Acad Sci U S A 106:2677-82
Zwart, Peter H; Afonine, Pavel V; Grosse-Kunstleve, Ralf W et al. (2008) Automated structure solution with the PHENIX suite. Methods Mol Biol 426:419-35
Shin, Dong Hae (2008) Preliminary structural studies on the MtxX protein from Methanococcus jannaschii. Acta Crystallogr Sect F Struct Biol Cryst Commun 64:300-3
Shin, Dong Hae (2008) A preliminary X-ray study of a refolded PTS EIIBfruc protein from Escherichia coli. Protein Pept Lett 15:630-2
Kim, Sung-Hou; Shin, Dong-Hae; Kim, Rosalind et al. (2008) Structural genomics of minimal organisms: pipeline and results. Methods Mol Biol 426:475-96

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