The goal of the JCSG Center for Innovative Membrane Protein Technologies (JCIMPT) is to develop and disseminate methods and technologies for structure-grade production of membrane proteins. JCIMPT is focusing on integral membrane proteins expressed in cell-free, baculovirus and mammalian cell systems. Close integration and collaboration with the Joint Center for Structural Genomics will provide access to databases, database tracking, and biophysical characterization technologies. In addition, the JCSG and the JCIMPT will collaborate on bacterial expression technologies. Miniaturization and automation are major components in our systematic investigation of membrane protein expression and the development of novel genetically engineered expression systems and sample preparation technologies. JCIMPT will synthesize and test a matrix of new molecules to stabilize integral membrane proteins. To develop the necessary methods and technologies, we have assembled a consortium of investigators that have extensive experience in both technology development and technology transfer for structural biology as well as for membrane protein production and structure determination by electron microscopy, NMR spectroscopy, and X-ray crystallography. A clear path for technology dissemination of the expression and sample preparation developments are in place through key collaborations with companies that already supply many structural biologists with the necessary tools to conduct their research. ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
5P50GM073197-03
Application #
7114421
Study Section
Special Emphasis Panel (ZGM1-CMB-0 (MP))
Program Officer
Chin, Jean
Project Start
2004-09-24
Project End
2009-07-31
Budget Start
2006-08-01
Budget End
2007-07-31
Support Year
3
Fiscal Year
2006
Total Cost
$2,651,143
Indirect Cost
Name
Scripps Research Institute
Department
Type
DUNS #
781613492
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Ishchenko, Andrii; Peng, Lingling; Zinovev, Egor et al. (2017) Chemically Stable Lipids for Membrane Protein Crystallization. Cryst Growth Des 17:3502-3511
Lamichhane, Rajan; Liu, Jeffrey J; Pauszek 3rd, Raymond F et al. (2017) Fluorophore Labeling, Nanodisc Reconstitution and Single-molecule Observation of a G Protein-coupled Receptor. Bio Protoc 7:
Eddy, Matthew T; Didenko, Tatiana; Stevens, Raymond C et al. (2016) ?2-Adrenergic Receptor Conformational Response to Fusion Protein in the Third Intracellular Loop. Structure 24:2190-2197
Rowe, Timothy B; Luo, Zhe-Xi; Ketcham, Richard A et al. (2016) X-ray computed tomography datasets for forensic analysis of vertebrate fossils. Sci Data 3:160040
Bennett, Brad C; Purdy, Michael D; Baker, Kent A et al. (2016) An electrostatic mechanism for Ca(2+)-mediated regulation of gap junction channels. Nat Commun 7:8770
Lamichhane, Rajan; Liu, Jeffrey J; Pljevaljcic, Goran et al. (2015) Single-molecule view of basal activity and activation mechanisms of the G protein-coupled receptor ?2AR. Proc Natl Acad Sci U S A 112:14254-9
Moeller, Arne; Lee, Sung Chang; Tao, Houchao et al. (2015) Distinct conformational spectrum of homologous multidrug ABC transporters. Structure 23:450-460
Fenalti, Gustavo; Abola, Enrique E; Wang, Chong et al. (2015) Fluorescence Recovery After Photobleaching in Lipidic Cubic Phase (LCP-FRAP): A Precrystallization Assay for Membrane Proteins. Methods Enzymol 557:417-37
Leung, Josephine H; Schurig-Briccio, Lici A; Yamaguchi, Mutsuo et al. (2015) Structural biology. Division of labor in transhydrogenase by alternating proton translocation and hydride transfer. Science 347:178-81
Kang, Yanyong; Zhou, X Edward; Gao, Xiang et al. (2015) Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser. Nature 523:561-7

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