Abstract

Theme E. Optical Biology: Improved Tools for in vivo Observation and QuantificationTheme Leader: Gratton.Other Project Faculty: Marsh, GrossOverview of the Optical Biology CoreThis Center is concerned with spatial dynamics at many levels. Recent improvements in microscopytechnology, probe development, image processing software, and specimen preparation have now madeit possible to monitor dynamic behaviors in biological systems with a degree of sensitivity and resolutiononce thought impossible. The great hurdle now is useful quantification of the information we can obtainin the form of images. The goal of the CCBS Optical Biology Core is to develop, apply, and makeavailable microscopy techniques for quantifying spatial dynamics in living systems. To this end, thecore is not simply a collection of equipment and personnel to help keep things running. It consists of specific tool application and tool development projects, led by Center investigators.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
1P50GM076516-01A1
Application #
7432210
Study Section
Special Emphasis Panel (ZGM1-CBCB-2 (SB))
Project Start
2007-07-01
Project End
2012-06-30
Budget Start
2007-08-01
Budget End
2008-07-31
Support Year
1
Fiscal Year
2007
Total Cost
$820,187
Indirect Cost

  Institution

Name
University of California Irvine
Department
Type
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697

  Publications

Lei, Jinzhi; Nie, Qing; Chen, Dong-Bao (2018) A single-cell epigenetic model for paternal psychological stress-induced transgenerational reprogramming in offspring. Biol Reprod 98:846-855
Huang, Jian; Chen, Long; Rui, Hongxing (2018) Multigrid Methods for A Mixed Finite Element Method of The Darcy-Forchheimer Model. J Sci Comput 74:396-411
Mahlbacher, Grace; Curtis, Louis T; Lowengrub, John et al. (2018) Mathematical modeling of tumor-associated macrophage interactions with the cancer microenvironment. J Immunother Cancer 6:10
McLelland, Bryce T; Lin, Bin; Mathur, Anuradha et al. (2018) Transplanted hESC-Derived Retina Organoid Sheets Differentiate, Integrate, and Improve Visual Function in Retinal Degenerate Rats. Invest Ophthalmol Vis Sci 59:2586-2603
Davey, Rhonda J; Digman, Michelle A; Gratton, Enrico et al. (2018) Quantitative image mean squared displacement (iMSD) analysis of the dynamics of profilin 1 at the membrane of live cells. Methods 140-141:119-125
Rackauckas, Christopher; Schilling, Thomas; Nie, Qing (2018) Mean-Independent Noise Control of Cell Fates via Intermediate States. iScience 3:11-20
Malacrida, Leonel; Gratton, Enrico (2018) LAURDAN fluorescence and phasor plots reveal the effects of a H2O2 bolus in NIH-3T3 fibroblast membranes dynamics and hydration. Free Radic Biol Med 128:144-156
Malacrida, Leonel; Rao, Estella; Gratton, Enrico (2018) Comparison between iMSD and 2D-pCF analysis for molecular motion studies on in vivo cells: The case of the epidermal growth factor receptor. Methods 140-141:74-84
Hedde, Per Niklas; Gratton, Enrico (2018) Selective plane illumination microscopy with a light sheet of uniform thickness formed by an electrically tunable lens. Microsc Res Tech 81:924-928
Kobylkevich, Brian M; Sarkar, Anyesha; Carlberg, Brady R et al. (2018) Reversing the direction of galvanotaxis with controlled increases in boundary layer viscosity. Phys Biol 15:036005

Showing the most recent 10 out of 404 publications