Abstract

The Tissue EM Core performs high-quality preservation of HIV-infected tissues from both humanized mouse model systems and actual human tissues from biopsies and explants. These samples are plastic-embedded, serially sectioned and imaged for tomographic analysis in either of two electron microscopes. Tomographic reconstructions provide three-dimensional, high-resolution studies of wild-type HIV within actual infected tissues and organs and may shed light on the fine structures involved in HIV budding and evasion from HAART therapy. The Core has most equipment necessary for processing, imaging and analyzing tissue samples. The core has effectively unlimited access to two tomography-ready electron microscopes: A FEI Tecnai T12 (120KeV) microscope for sections <200nm in the Caltech Broad Center EM Suite (see EM Tomography Core, Grant Jensen Director) and a newly retrofitted Tecnai TF30-FEG (300KeV) microscope for 300-400nm sections located in the Caltech Material Sciences Department.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
5P50GM082545-07
Application #
8538439
Study Section
Special Emphasis Panel (ZRG1-AARR-K)
Project Start
Project End
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
7
Fiscal Year
2013
Total Cost
$282,827
Indirect Cost

  Institution

Name
University of Utah
Department
Type
DUNS #
009095365
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112

  Publications

Coey, Aaron T; Larsen, Kevin P; Choi, Junhong et al. (2018) Dynamic Interplay of RNA and Protein in the Human Immunodeficiency Virus-1 Reverse Transcription Initiation Complex. J Mol Biol 430:5137-5150
Swulius, Matthew T; Nguyen, Lam T; Ladinsky, Mark S et al. (2018) Structure of the fission yeast actomyosin ring during constriction. Proc Natl Acad Sci U S A 115:E1455-E1464
Hammond, John A; Zhou, Li; Lamichhane, Rajan et al. (2018) A Survey of DDX21 Activity During Rev/RRE Complex Formation. J Mol Biol 430:537-553
Wang, Haoqing; Barnes, Christopher O; Yang, Zhi et al. (2018) Partially Open HIV-1 Envelope Structures Exhibit Conformational Changes Relevant for Coreceptor Binding and Fusion. Cell Host Microbe 24:579-592.e4
Pastuzyn, Elissa D; Day, Cameron E; Kearns, Rachel B et al. (2018) The Neuronal Gene Arc Encodes a Repurposed Retrotransposon Gag Protein that Mediates Intercellular RNA Transfer. Cell 172:275-288.e18
Wagner, Jonathan M; Christensen, Devin E; Bhattacharya, Akash et al. (2018) General Model for Retroviral Capsid Pattern Recognition by TRIM5 Proteins. J Virol 92:
Donaldson, G P; Ladinsky, M S; Yu, K B et al. (2018) Gut microbiota utilize immunoglobulin A for mucosal colonization. Science 360:795-800
Bailey, Lucas J; Sheehy, Kimberly M; Dominik, Pawel K et al. (2018) Locking the Elbow: Improved Antibody Fab Fragments as Chaperones for Structure Determination. J Mol Biol 430:337-347
Pak, Alexander J; Voth, Gregory A (2018) Advances in coarse-grained modeling of macromolecular complexes. Curr Opin Struct Biol 52:119-126
Redman, Joseph S; Francis, J Nicholas; Marquardt, Robert et al. (2018) Pharmacokinetic and Chemical Synthesis Optimization of a Potent d-Peptide HIV Entry Inhibitor Suitable for Extended-Release Delivery. Mol Pharm 15:1169-1179

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