Abstract

The Specialized Population Research Center proposed in this application targets a synthesis of hypotheses and modern biomedical techniques in a continuum of disciplines in reproductive sciences from molecular biology to physiology. Close integration of principles, resources and expertise is fundamental. The work includes ten projects which fall into the two broad categories of neuroscience and early development; I, a study of TGF-beta, II, an analyses of mammalian spermatogenesis; III, a study of hap26 expression in Drosophila, IV, the study of the GnRH pulse generator in primates, V, a study of the prenatal expression of growth factor genes and receptors; VI, a study of GnRH networks in the rhesus brain by neuroanatomical methods,; VII, analysis of placental CRH in pregnant baboons, VIII, experiments with steroid/growth factors in neurodevelopment; IX, study of sex steroid regulation of brain POMC and LH and X, an analysis of proto-oncogene expression during mammalian gametogenesis. Five biomedical cores are organized to expedite technical usage of a primate colony, molecular cytology/histology techniques, radioimmunoassay of steroids and proteins, cell and hybridization culture, and a molecular biology facility.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Specialized Center (P50)
Project #
5P50HD005077-20
Application #
3106114
Study Section
Population Research Committee (HDPR)
Project Start
1975-04-30
Project End
1994-03-31
Budget Start
1990-04-01
Budget End
1991-03-31
Support Year
20
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
Columbia University (N.Y.)
Department
Type
Schools of Medicine
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10027

  Publications

Rhee, K; Wolgemuth, D J (1997) The NIMA-related kinase 2, Nek2, is expressed in specific stages of the meiotic cell cycle and associates with meiotic chromosomes. Development 124:2167-77
Zhang, Q; Ahuja, H S; Zakeri, Z F et al. (1997) Cyclin-dependent kinase 5 is associated with apoptotic cell death during development and tissue remodeling. Dev Biol 183:222-33
Ravnik, S E; Wolgemuth, D J (1996) The developmentally restricted pattern of expression in the male germ line of a murine cyclin A, cyclin A2, suggests roles in both mitotic and meiotic cell cycles. Dev Biol 173:69-78
Rhee, K; Wolgemuth, D J (1995) Cdk family genes are expressed not only in dividing but also in terminally differentiated mouse germ cells, suggesting their possible function during both cell division and differentiation. Dev Dyn 204:406-20
Winer, M A; Wolgemuth, D J (1995) The segment-specific pattern of A-raf expression in the mouse epididymis is regulated by testicular factors. Endocrinology 136:2561-72
Wu, S; Wolgemuth, D J (1995) The distinct and developmentally regulated patterns of expression of members of the mouse Cdc25 gene family suggest differential functions during gametogenesis. Dev Biol 170:195-206
Ravnik, S E; Rhee, K; Wolgemuth, D J (1995) Distinct patterns of expression of the D-type cyclins during testicular development in the mouse. Dev Genet 16:171-8
Mehta, N D; Don, J; Zinn, S A et al. (1994) Proenkephalin gene expression in testicular interstitial cells is down-regulated coincident with the appearance of pachytene spermatocytes. Endocrinology 135:1543-50
Don, J; Winer, M A; Wolgemuth, D J (1994) Developmentally regulated expression during gametogenesis of the murine gene meg1 suggests a role in meiosis. Mol Reprod Dev 38:16-23
Chapman, D L; Wolgemuth, D J (1994) Regulation of M-phase promoting factor activity during development of mouse male germ cells. Dev Biol 165:500-6

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